摘要
目的研究苦参碱对人雄激素非依赖性前列腺癌细胞株PC-3增殖、细胞周期及细胞凋亡的影响。方法不同浓度苦参碱作用PC-3细胞后,应用MTT法观察苦参碱对PC-3细胞增殖的影响,流式细胞仪研究苦参碱对PC-3细胞周期的影响,TUNEL法、Annexin V/PI双染分析苦参碱对PC-3细胞凋亡的影响。结果苦参碱对PC-3细胞的生长具有抑制作用,与对照组相比有统计学差异(P<0.05)。苦参碱对PC-3细胞的增殖抑制作用呈现时间及剂量依赖性关系。苦参碱的最佳药物浓度为1.0g/L,最佳作用时间为48h。流式细胞仪分析显示不同浓度苦参碱均可使PC-3细胞阻滞于G0/G1期,随着苦参碱浓度的增加,G0/G1期细胞所占的比例增加,与对照组相比有统计学差异(P<0.01)。TUNEL法、Annexin V/PI分析显示不同浓度苦参碱均可诱导PC-3细胞凋亡,其早期凋亡率及晚期凋亡率随着苦参碱浓度的增加而升高,呈现剂量依赖性关系(P<0.05,P<0.01)。结论苦参碱在体外可抑制PC-3细胞的增殖,具有时间和浓度依赖关系。抑制增殖和细胞周期阻滞与G0/G1期相关。苦参碱可诱导PC-3细胞凋亡,与苦参碱的作用浓度及时间有关。
Objective To investigate the effects of matrine on the proliferation, cell cycle, apoptosis of androgen independent prostate cancer （AIPC） cell line PC-3 in vitro. Methods PC-3 cells were treated by different concentrations of matrine. The inhibitory effect of matrine on the proliferation of PC-3 cells was observed by MTT method. The cell cycle of PC-3 cells treated by matrine was observed by flow cytometric assay. Apoptosis of PC-3 cells induced by matrine was determined with TUNEL and Annexin V/PI staining assay. Results MTT test showed that PC-3 cells treated by matrine had an inhibitory effect, which was significantly different from that of controls （P 〈0.05）. Matrine markedly inhibited cell proliferation of PC-3 in dose- and time-dependent manners. The optimal dose of matrine which inhibited PC-3 cells was 1.0 g/L and the best time of inhibition was at 48 h. Flow cytometric assay showed that the PC-3 cells treated by different doses of matrine were arrested in cell cycle progression at G0/G1 phase. The cell ratio at G0/G1 phase increased with the increase of matrine dose, which was significantly different from that of controls （P 〈 0.01）. TUNEL and Annexin V/PI staining assay showed that matrine could obviously induce the apoptosis of PC-3 cells. The ratio of early and late apoptosis of PC-3 ceils treated by matrine was increased in a dose-dependent manner （P 〈 0.05, P 〈 0.01）. Conclus the proliferation of PC-3 cells in dose- and time-dependent manners in vitro. The associated with arresting cell cycle at G0/G1 phase. Matrine could induce the apoptosis of associated with the dose and actioli time of matrine. on Matrine could inhibit inhibition mechanism was PC-3 cells, which might be
出处
《西安交通大学学报（医学版）》
CAS
CSCD
北大核心
2009年第4期510-514,520,共6页
Journal of Xi’an Jiaotong University（Medical Sciences）
基金
陕西省科学技术研究发展计划项目[No.2007k09-09(9)]~~
