摘要
以耐旱性不同的2个番茄品种为试材,采用营养液水培法,研究了钙、钙调素拮抗剂W7〔N-(6-aminohexyl)-5-chloro-1-naphthalene sulfonamide〕预处理对聚乙二醇模拟干旱胁迫下番茄幼苗抗氧化系统的影响。结果表明,钙调素拮抗剂W7浸种处理显著提高了番茄幼苗丙二醛(MDA)、H2O2含量和O2.产生速率,抑制了超氧化物歧化酶(SOD)、过氧化物酶(POD)活性,加剧了抗坏血酸(AsA)和还原型谷胱甘肽(GSH)的破坏;而Ca2+处理显著降低了PEG胁迫下番茄幼苗MDA、H2O2含量和O2.产生速率,提高了SOD、POD活性,减轻了胁迫对AsA和GSH的破坏。相同处理条件下,耐旱性强的品种‘毛粉802’较耐旱性弱的品种‘皇冠’伤害程度轻,Ca2+-CaM信号系统对缓解番茄PEG胁迫具有重要作用。
The effect of Ca^2 ＋ and calmodulin antagonist W7 on antioxidant systems of tomato （ Lycopersicon esculenturn M. ） seedlings under drought stress induced by 10% polyethylene glycol （PEG - 6000） was studied in hydroponics culture. The results showed that pretreated seedlings with calmodulin antagonist W7 under PEG stress had lower SOD and POD activities, and higher production rate of O2^-, MDA and H2O2, in addition, aggravated the damage of AsA and GSH; On the contrary, Ca^2＋ treatment enabled the seedlings to keep relatively high activities of SOD and POD, lower production rate of O2^- and MDA content, at the same time, alleviated the accumulation of AsA and GSH. These results indicated that Ca^2＋ - CaM signal transduction might regulate resistance of tomato seedlings to PEG stress of tomato seedlings by affecting the activity of some antioxidant enzymes and the content of antioxidant substance. The experiment shows that Ca^2＋ - CaM signal system plays an important regulating role in alleviating PEG stress induced by 10% polyethylene glycol （PEG- 6000） in seedlings.
出处
《干旱地区农业研究》
CSCD
北大核心
2009年第4期111-115,135,共6页
Agricultural Research in the Arid Areas
基金
国家"十一五"攻关项目(2007BAD79B04)
