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酵母表面展示技术在蛋白质工程中的应用 被引量:1

Application of Yeast Cell-surface Display for Protein Engineering
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摘要 酵母细胞表面展示技术目前已成为蛋白质工程研究的重要工具,利用此技术可以鉴定蛋白间的相互作用、提高蛋白的亲和力和特异性、增加蛋白的稳定性和表达水平、绘制功能性抗原位图、固定化表达具有生物活性的蛋白和酶等,此项技术的运用代表着蛋白质工程研究中的最新进展。 The technology of yeast cell-surface display has been an important tool for researching protein engineering. It can be used to identify protein-protein interaction, improve affinity and specificity of proteins, increase stability and expression level, map functional protein epitopes and immobilize proteins and enzymes. The utilization of yeast cell-surface display for protein engineering represents a significant recent advance.
出处 《生物技术通报》 CAS CSCD 北大核心 2009年第8期63-66,共4页 Biotechnology Bulletin
基金 国家高技术研究发展计划("863"项目)(2006AA10Z316)
关键词 蛋白质工程 酵母细胞表面展示 酶固定化 亲和力 蛋白表位 Protein engineering Yeast cell-surface display Enzyme immobilization Affinity Protein epitope
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参考文献27

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