摘要
目的:采用HPLC方法建立不同产地夏枯草属药材指纹图谱,并对其进行聚类分析评价。方法:建立高效液相指纹图谱分析方法,色谱柱为Alltima C18柱(4.6mm×250mm,5μm);流动相为甲醇-0.5%冰醋酸溶液梯度洗脱;流速为1.0ml/min;检测波长:280nm;进样量20μl;柱温为30℃。测定了3个品种不同产地的夏枯草属药材指纹图谱,并对其聚类分析。结果:不同产地药材指纹图谱特征有明显差异,湖北、安徽、江西、贵州、广西、四川产地药材归为1类;河南、江苏、浙江3个产地夏枯草归为1类。结论:建立的指纹图谱能明显的反应出各个产地药材的特征,且聚类分析有效的区分药材的归属性。
Objective： To establish the HPLC fingerprint of Prunella Species from different places and evaluate it by cluster analysis. Methods： The analysis method of HPLC fingerprint was established. Alltima C18 （ 4.6mm×250mm, 5μm ） was utilized for separation. The mobile phase consisted of methanol （A） and 0.5% glacial acetic acid （B） with a flow-rate of 1.0ml/min. The detection wavelength was 280nm and the injection volume was 20μl. The column temperature was 30℃. The HPLC fingerprints of Prunella Species from three different places were determined and analyzed by SPSS. Results： There is a big difference among fingerprints of Prunella from different places. Prunella from Hubei, Anhui, Jiangxi, Guizhou, Guangxi, Sichuan were divided into a class; Henan, Jiangsu, Zhejiang were divided into another class. Conclusion： the eatsblished fingerprint showed characteristics of Prunella Species from different places obviously, and the attribute of Prunella Species can be distinguished effectively by cluster analysis method.
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2009年第9期1216-1219,共4页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
江苏省自然科学基金(No.BK2006155)
江苏省中医药领军人才项目(No.2006)
关键词
夏枯草
山菠菜
硬毛夏枯草
HPLC
聚类分析
组分结构理论
Prunella vulgaris L.
P. asiatica Nakai
P. hispida Benth.
HPLC
Cluster analysis
Composition- structure theory
