摘要
目的研究胃癌组织中缺氧诱导因子-1（HIF-1）和环氧合酶-2（COX-2）表达的临床病理学意义，探讨其与淋巴结转移、微血管密度（MVD）的关系。方法采用免疫组织化学法检测64例胃癌和15例正常胃黏膜组织中HIF-1α和COX-2的表达，用CD34抗体标记微血管并计数相应的微血管密度（MVD）。结果64例胃癌组织中HIF-1α和COX-2的阳性表达率分别为67．19％和78．13％，HIF-1α与COX-2在胃癌组织中的表达水平明显高于正常胃组织中的表达水平（P〈0．05）。HIF-1α和COX-2的表达与胃癌的临床TNM分期、浸润深度和淋巴结转移有关（P〈0．05）；与患者的年龄、性别和组织学分级无关。HIF-1α和COX-2之间存在显著相关性（L=0．584，P〈0．01）。HIF-1俚阳性表达组的MVD均值（36．78±10．46）明显高于阴性表达组（17．92±3．71），COX-2阳性表达组的MVD均值（37．59±10．75）明显高于阴性表达组（18．43±4．15），HIF-1α、COX-2的表达与MVD之间呈正相关（t=0．624，P〈0．01，0．697，P〈0．01）。结论HIF-1α和COX-2在胃癌的发生、发展中起重要作用，并且在促进肿瘤血管形成过程中两者可能起协同作用，联合检测可作为判断胃癌恶性程度和预后的指标。
Objective To study the expression of hypoxia inducible factor-lalpha （HIF-1α） and cyclooxygenase-2 （COX-2） in gastric carcinoma and its correlation with lymph node metastasis and microvascular density （MVD）. Methods HIF-1α and COX-2 expression were detected by immunohistochemistry in 64 cases of gastric carcinoma and 15 cases of normal gastric mucosa tissues, MVD of the section marked with CD34 antibody was calculated. Results The positive rates of HIF-1α and COX-2 in 64 cases of gastric carcinoma were 67.19 % and 78.13 % respectively. The expressions of HIF-1α and COX-2 were significantly higher in gastric carcinoma than in normal gastric mucosa tissue（P 〈 0.05）. The expressions of HIF-1α and COX-2 in gastric carcinoma were significantly associated with TNM stage, invasive depth and lymph node metastasis （P〈 0.05） except for patient＇s age, sex and tumor grade.There were positive correlation between HIF-1α expression with COX-2 （rs =0.584, P 〈 0.01）. The MVD of cases with positive HIF-1α expression（36.78±10.46） was significantly higher than those without HIF-lct expression（17.92±3.71）, the MVD of cases with positive COX-2 expression（37.59±10.75） was also higher than those without COX-2 expression （18.43±4.15）. The expression of HIF-1α and COX-2 was associated with MVD（rs =0.624, 0.697, P〈 0.01）. Conclusion HIF-1α and COX-2 play important roles in the pathway of carcinogenesis and progression of gastric carcinoma, and they may contribute to tumor angiogenesis through the same way, thus combined detection of HIF-1α and COX-2 may be recommended for diagnosis of gastric cancer and estimation of prognosis.
出处
《肿瘤研究与临床》
CAS
2009年第8期453-456,共4页
Cancer Research and Clinic
关键词
胃肿瘤
免疫组织化学
缺氧诱导因子1
Α亚基
环氧合酶2
微血管密度
淋巴转移
Stomach neoplasms
Immunohistochemistry
Hypoxia-inducible factor-1, alpha subunit
Cyclooxygenase-2
Microvascular density
Lymphatic metastasis
