摘要
以乙醇从脱脂玉米蛋白粉中萃取所得的醇溶蛋白为底物，以碱性蛋白酶为催化剂，通过水解反应制备玉米醇溶蛋白肽，经精制冻干后得到肽含量为85．73％的玉米醇溶蛋白肽固体。以α-生育酚为阳性对照，研究了玉米醇溶蛋白肽及其分离纯化组分对亚油酸自氧化的抑制作用。结果表明，玉米醇溶蛋白肽及其分离组分都具有抑制亚油酸自氧化的作用，抑制效果随其质量浓度的增大而增强。经Sephedax G—15葡聚糖凝胶层析分离所得组分2抑制亚油酸自氧化的效果最好，相对分子质量主要集中在200—800Da之间。
Zein extracted from defatted corn gluten meal by ethanol was hydrolyzed with an alkaline protease. The hydrolysate was refined and freeze - dried to obtain zein peptide powder containing 85.73% peptide. Using α - tocopherol as the positive control, the antioxidant abilities of zein peptide and its separated fractions were examined by investigating their inhibiting degree to linoleic acid autooxidation. The data revealed that the zein peptide and its separated fractions exhibited a potent inhibiting effect on linoleic acid autooxidation,and the inhibiting effect related positively to its concentrations. The activest fraction was fraction 2 separated by Sephadex G - 15 method. The relative molecular mass distributions of the fraction 2 were mostly focused on between 200 Da and 800 Da.
出处
《中国油脂》
CAS
CSCD
北大核心
2009年第9期19-23,共5页
China Oils and Fats
