摘要
目的:利用分子生物技术构建色氨酸羟化酶(TPH2)启动子调控红色荧光蛋白(red fluorescent prote in,RFP)转基因细胞,以筛选吴茱萸汤及其各组成药物对TPH2启动子的调控活性,探讨其治疗偏头痛可能的分子靶点。方法:构建TPH2启动子调控红色荧光蛋白表达的转基因细胞系,观察统计不同给药组中细胞荧光强度的变化及发光细胞数量的变化。结果:通过荧光细胞计数发现吴茱萸汤、吴茱萸、生姜、大枣水提物不同透析部分在不同浓度上对TPH2启动子转录活性具有不同的调控作用。结论:吴茱萸汤治疗偏头痛的可能分子靶点与激活TPH2表达有关。TPH2启动子调控红色荧光蛋白表达细胞模型可用于对以此为靶点的药物筛选和活性评价。
Objective： To screen the active component of Wuzhuyutang （WZYT, Evodiae prescription） and investigate the regulatory effects of the components in WZYT on the TPH2 promoter, and to explore the possible molecular mechanism of WZYT on migraine. Method： By transfecting a TPH2 promoter regulating Red Fluorescent Protein expressing plasmid into PC12 cell, the global fluorescence intensities and calculations of fluorescent cells after components treatment were statistically evaluated. Result： Different regulatory effects of different components in WZYT with different concentrations on TPH2 promoter were observed. Conclusion： TPH2 promoter drove Red Fluorescent Protein expressing cell line can be used as system screening components targeting TPH2 promoter activity. The possible mechanism of WZYT on migraine may due to its stimulating effects on TPH2 promoter, and promote the synthesis and release of 5-HT in cerebral.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2009年第17期2261-2264,共4页
China Journal of Chinese Materia Medica
基金
国家"十一五"科技支撑计划(2006BAI08B03-09)
中国博士后科学基金(20080440418)
