摘要
采用ＭＴＴ比色法检测细胞增殖情况，用放免法（ＲＩＡ）测定胰岛素、胰高血糖素和胃泌素含量，氨基己糖含量用Ｎｅｕｈａｕｓ法测定，以期观察五肽胃泌素和生长抑素对体外培养的人胃癌细胞ＨＧＣ８０３和ＨＧＣ８２３生长的调节作用。结果显示，胃泌素对两株胃癌细胞增殖均有促进作用，以１×１０－５ｍｏｌ／Ｌ浓度最为明显，并使胃癌细胞氨基己糖含量明显增加（７．５８±０．６６ｖｓ４．２±０．３９ｐｇ／细胞，Ｐ＜０．０５））。生长抑素对两株胃癌细胞增殖均有抑制作用。以１×１０－６ｍｏｌ／Ｌ浓度最为明显，同时还抑制胃癌细胞分泌胃泌素和胰高血糖素的能力，并使胃癌细胞氨基己糖含量明显减少（２．６２±０．２９ｖｓ４．２±０．３９ｐｇ／细胞，Ｐ＜０．０５）。胃肠激素可调节胃癌细胞的生长，但尚有许多问题待阐明。
In order to observe the regulative effects of pentagastrin (PG) and somatostatin (SS) on the growth of two human gastric cancer cell lines (HGC803 and HGC823) in vitro, we observed the effects of PG and SS on proliferation of human gastric cancer cells by means of MTT. The contents such as gastrin, insulin, and glucagon were determined by radioimmunoassay (RIA), and the hexosamine content was determined by Neuhaus' method. The results showed that the growth of the two human gastric cancer cell lines were obviously promoted by PG. On the contrary, the growth and secretion of gastrin and glcagon were inhibited by SS. In addition, the hexosamine content of human gastric cancer cells was significantly increased by PG (7.58±0.66 versus 4.20±0.39 pg/cell, (P<0.05). But the hexosamine content was decreased by SS (2.62±0.29 versus 4.20±0.39 pg/cell, P<0.05). These findings indicate that the growth of gastric cancer cells is regulated by PG and SS, nevertheless a host of problems need to be elucidated.
出处
《华西医科大学学报》
CSCD
1998年第3期292-294,共3页
Journal of West China University of Medical Sciences
