摘要
目的检测PTEN基因在子宫内膜异位症(EMs)和正常子宫内膜中的表达,探讨其在EMs发生、发展中的作用。方法收集45例EMs患者术后异位内膜及在位内膜标本(其中包括子宫腺肌病和卵巢子宫内膜异位症),并收集非子宫内膜异位症的子宫内膜标本40例,用SP免疫组织化学方法检测PTEN在其中的表达。结果PTEN主要表达于子宫内膜腺上皮细胞的胞核中,在间质细胞中有少量表达。PTEN在非子宫内膜异位症的子宫内膜中高表达,为92.5%;在异位内膜中的表达水平明显降低,为37.8%;而PTEN在在位内膜中的表达为68.9%,介于正常内膜与异位内膜之间,两两比较差别有显著性。结论PTEN表达降低和缺失可能是EMs发生、发展的机制之一,PTEN可能参与了月经周期的调节。
Objective In order to explore whether PTEN was involved in the pathogenesis and progression of endometriosis, we evaluated phosphatase and tension homologue deleted on chromosome 10（PTEN） in endometriosis and the endometrium of non-- endometriosis. Methods The expression of PTEN in eutopic and ectopic endometrium obtained from 45 patients with endometriosis, including 30 ovarian endometriesis and 15 adenomyesis, was analyzed by immunohistochemical method. The endometrium of non-- endometriosis taken from 40 cases as controls.Results The expression of PTEN was found at epithelial cell nuclei, a little was at interstitial cells. PTEN in the endometrium of non--endometriesis showed high expression（92.5%）,in the ectopic endometrium was significantly decreased（37.8%）, in the eutopic endometrium was between them above（68.9%）.Compared between the ectopic, eutopic endometrium and the endometrium of non--endometriosis there were statistically different.Conclusion The express decrease or deletion of PTEN is possibly one of the pathogenesis of endometriosis. PTEN may be regulates the menstrual cycle.
出处
《中外医疗》
2009年第25期151-152,155,共3页
China & Foreign Medical Treatment
