摘要
用溶液法合成了镍(Ⅱ)与2-(2-吡啶)苯并咪唑(HPBM),咪唑的混配配合物,其分子式为[Ni(C12N3H9)2(C3H4N2).Cl].(ClO4).在接近生理条件pH=7.2下,研究它与DNA作用的电子吸收光谱、荧光光谱,求得配合物与DNA的结合常数,研究了配合物对EB-DNA体系的Scatchard图,结果表明配合物与DNA作用既存在部分插入结合模式又存在静电结合模式.
This paper introduces that the microwave synthesis of 2 - （2 -pyridyl） -benzimidazole and solvothermal reaction of Ni （II） complex with 2 - （2 - pyridyl） - benzimidazole and imidazole,the structures of the complex may be [Ni（C12N3H9）2（C3H4N2）·Cl]·（ClO4）. The interaction of the complex and DNA has been investigated by using UV spectra and fluorescent spectra. The emission intensity was quenched as the complex was added to the EB - DNA system. The apparent binding constants of the complex and DNA was obtained at physiological pH （ pH = 7.2）. The results suggest that the interaction of Ni （II） complex and DNA belong to both partial intercalation and static electricity attraction mode.
出处
《南华大学学报（自然科学版）》
2009年第3期46-52,共7页
Journal of University of South China：Science and Technology
基金
湖南省自然科学基金资助项目(02JJY2018
06JJ30004
07JJ5005)
