摘要
采用循环伏安（CV）法在金电极表面电聚合一层铁氰酸钴（CoHCF），利用花四甲酸二酐衍生物（PTC-NH2）成膜能力强又带有许多氨基功能团的特点将其固定在CoHCF表面，再通过静电吸附和共价键合作用将纳米金和辣根过氧化物酶（HRP）的复合物制得过氧化氢生物传感器VHRP-Au／PTC-NH2／CoHCF／Au]．考察了该传感器对过氧化氢的电化学特性，实验表明，该传感器具有良好的生物催化活性，响应电流与过氧化氢浓度在2．0×10^-6-1．6×10^-3mol／L范围呈良好的线性关系，检出限为7．28×10^-7mol／L．
The cobalt hexacyanoferrate （CoHCF） film modified-gold electrode was successfully prepared by cyclic voltammetry （CV）. Then, the derivative of 3,4,9,10-perylenetetracarboxylic dianhydride （PTCNH2）, which contains rich amino groups and owns good film-forming properties, was dropped on the surface of CoHCF. Finally, the composite of nano-gold and horseradish peroxidase （HRP） was immobilized on PTC-NH2 surface via the effect of electrostatic adsorption and covalent bonding to construct a new H2O2 biosensor, denoted as HRP-Au/PTC-NH2/CoHCF/Au electrode. The electrocatalytic behavior of the modified electrode to H2O2 was investigated by cyclic voltammetry and chronoamperometry. The experiment results showed that the modified electrode had excellent biocatalytic activity for the reduction of H2O2. The linear range of this biosensor was 2.0×10^-6-1.6× 10^-3 mol/L with a detection limit of 7.28× 10^-7 mol/L.
出处
《西南大学学报（自然科学版）》
CAS
CSCD
北大核心
2009年第9期97-101,共5页
Journal of Southwest University(Natural Science Edition)
基金
国家自然科学基金资助项目(20675064)
重庆市自然科学基金资助项目(CSTC-2005BB4100)
关键词
铁氰酸钴
藐四甲酸二酐衍生物
纳米金
辣根过氧化物酶
生物传感器
cobalt hexacyanoferrate
derivative of 3,4,9, 10-perylenetetracarboxylic dianhydride
nanogold
horseradish peroxidase~ biosensor
