摘要
对热处理茎尖组织培养法繁育苹果无病毒苗木进行实验研究。北斗、王林等5个苹果品种的盆栽苗经38℃±1℃恒温处理21～35天,取处理苗茎尖灭菌后,切取0.10～0.15cm长的生长点置MS+1.5ppm6-BA+0.2ppmIBA培养基中,培养40天后,剪取分化苗转入各品种适宜的生长分化培养基中,继代培养2～3次,可获足量的试管苗。取少量组培苗研磨成汁,用酶联免疫吸附法(ELISA)和摩擦接种草本指示植物进行病毒检测,北斗、王林、红乔纳金和高岭4品种均不带苹果褪绿叶斑病毒(ACLSV)和苹果茎沟病毒(ASGV),早富士品种仍带有茎沟病毒(ASGV)。剪取组织培养苗,用100ppmIBA处理12小时后,置1/2MS+0.1ppmGA+1.5ppmIAA+0.5g/L活性炭各品种皆适宜的生根培养基中培养,生根率达72％～98％。生根苗在5～30℃的温室内自然光下闭口驯化4周,开口驯化1周,植入普通腐质土中,成活率达90％～100％。
The way to propagate virus-free apple clones rapidly by tissue culture of heat therapy tips is reported in the paper. The length of the tips cutted from young apple trees, which had grown for 21 to 35 days in the temperature of 38±1℃, is given. Five growth mediums,a productive root method and the way to tame root seedling for planting in general soil are described. The article also introduces the method and the result of detection of latent viruse in apple by herbaceous indicators and indirect enzyme-linked immunosorbent assay(ELISA).
出处
《莱阳农学院学报》
1990年第4期305-309,共5页
Journal of Laiyang Agricultural College
关键词
苹果
组织培养
无病毒苗木
apple
heat therapy
tips
tissue culture
detection of viruse
viruse—free clones
