摘要
利用正交试验设计,用乙醇回流法提取广西刺楸茎皮中的总皂苷,并采用大孔吸附树脂和正丁醇分别对其进行分离,通过纯度分析和得率计算,探索其主皂苷成分的最佳提取工艺。实验结果表明采用大孔吸附树脂法分离主皂苷成分得率为1.32%,而采用正丁醇法分离其得率为1.05%;且大孔吸附树脂法的产品外观颜色优于正丁醇法。采用大孔吸附树脂法提取分离该植物的主皂苷成分,成本低,操作简便易行且具有非常强的实用性。
Using orthogonal experiment design, the total saponin constituents were obtained by refluxing extraction with alcohol and separated by macroporous adsorption resin and n-Butyl alcohol from the stem bark of Kalopanax septemlobus. According to the purity analysis and the yield, the extraction process was optimized. The results showed that the main saponin constituents were gained with a yield of 1.32% by using macroporous adsorption resin but 1.05% by using n-Butyl alcohol. The former was more efficient than the latter on both yield and color. The optimal process with isolation by macroporous adsorption resin is cheap, simple and practical.
出处
《中药材》
CAS
CSCD
北大核心
2009年第9期1457-1459,共3页
Journal of Chinese Medicinal Materials
基金
广西自然科学基金资助(桂科计字[2004]47号)
关键词
大孔吸附树脂
正丁醇
正交试验
主皂苷成分
Macroporous adsorption resin
n-Butyl alcohol
Orthogonal experiment
Main saponin constituents
