摘要
选择长江三角洲地区一处长期受到有机农药污染的浅层含水层,采集水样,采用不依赖于培养的16S rDNA序列分析方法,对该污染地下水中的微生物群落特征进行了分析和鉴定。结果表明,这种分析方法完全可行,该有机污染含水层具有较好的微生物多样性,且可能有一种未经鉴定的新菌种。
A shallow aquifer, located in Yangtze River Delta and polluted by organic substances for long time, was chosed to study the microbial diversity and the relations of genus and species, adopting the 16S rDNA sequencing method, a microbial molecular ecology technique that analyzed microbial community and without any cultivation. Experimental results showed, the 16S rDNA sequencing method was well successful. The shallow aquifer suffering from long-time organic contamination had a good microbial diversity, while the bacterium belonging to an unknown genus was probably discovered. The present study builds a practical method for the identification of microbial diversity in the polluted groundwater and provides scientifical basis, to some extent, for the further studies on the biological remediation technology of groundwater polluted by organic compouds.
出处
《农业环境科学学报》
CAS
CSCD
北大核心
2009年第9期1898-1902,共5页
Journal of Agro-Environment Science
基金
国家自然科学青年基金(40502024)
国家杰出青年基金(40725010)
污染控制与资源化研究国家重点实验室开放基金(PCR200406)
浙江工业大学环境工程"重中之重"学科开放基金(20080206)
