摘要
探讨骨髓基质细胞诱导的神经干细胞移植至海人酸(kanic acid,KA)致痫大鼠海马后对学习记忆障碍的改善作用,从而为干细胞移植治疗癫痫提供理论依据.体外分离大鼠骨髓基质细胞,并在特定条件下培养、诱导分化为神经干细胞.在海马内注射KA建立大鼠癫痫模型,之后把诱导的神经干细胞移植至KA致痫鼠的海马内,用Morris水迷宫测试大鼠学习记忆能力,用PCR方法检测海马内NSE、c-fos和SEZ-6基因表达,采用Western blot技术检测海马内NSE、C-FOS和SEZ-6蛋白表达.结果显示:骨髓基质细胞成功诱导分化为神经干细胞,诱导分化的神经干细胞移植到脑内可明显改善大鼠的学习记忆障碍;移植组大鼠海马内NSE、c-fos和SEZ-6基因表达下调,NSE、C-FOS和SEZ-6蛋白表达亦下调,与阳性对照组及假移植组比较,差异显著(P<0.05).说明骨髓基质细胞诱导的神经干细胞移植至KA致痫大鼠海马后对学习记忆障碍有明显的改善作用,其机制可能与NSE、c-fos、SEZ-6基因和蛋白的表达下调相关.
In order to research the recovery effect of neural stem cells （NSCs） derived from bone marrow stromal cells （BMSCs） to transplant into hippocampus of rats with epilepsy induced by kanic acid on their learning and memory dysfunction and approach the theory about NSCs transplantation treating epilepsy, the BMSCs were segregated, cultured and differentiated into NSCs in vitro at first, and secondly the rat models of epilepsy were established with kanic acid. At last, the NSCs derived from BMSCs were transplanted into hippocampus of rats with epilepsy and the learning and memory ability of rats were tested with Morris maze. The expression levels of NSE, c-fos and SEZ-6 genes were detected by RT-PCR and at the same time, the protein levels of NSE, C-FOS and SEZ-6 were measured by Western blot. The results display that the BMSCs were successfully induced into NSCs in vitro. The impairment of learning and memory of epileptic rats was significantly improved after the NSCs were transplanted into their hippocampus. The mRNAs expression levels of NSE, c-fos, SEZ-6 and their proteins levels were down-regulated in hippocampus tissue, difference being obvious in transplantation group compared with positive control group and sham-graft group （P〈0.05）. It was indicated that the dysfunction of learning and memory of rats with epilepsy was markedly improved after transplantation of NSCs derived from BMSCs into hippocampus, and the mechanism might be correlated with NSE, c-los and SEZ-6 expression down-regulation.
出处
《浙江大学学报（农业与生命科学版）》
CAS
CSCD
北大核心
2009年第5期523-531,共9页
Journal of Zhejiang University:Agriculture and Life Sciences
基金
吉林省吉林市科技局科研基金资助项目(2007087)
关键词
骨髓基质细胞
神经干细胞
颞叶癫痫
学习记忆
bone marrow stromal cells
neural stem cells
temporal lobe epilepsy
learning and memory
