摘要
聚β羟基丁酸酯（ＰＨＢ）是微生物合成的一种以颗粒状态存在于细胞中的高分子聚合物，由于它具有生物可降解性、生物相容性等特性，在医学上具有独特而广阔的应用前景。从微生物细胞中分离ＰＨＢ的方法有溶剂萃取法［１］、化学试剂法［２４］和酶法［５］。目前工...
A combined SDS NaClO method to recover PHB from recombinant E.coli HMS 174(pTZ18u PHB) was studied.The adding order of SDS and NaClO affected the result of this method and the reason was analyzed.A new process that the cell was treated by SDS for 10min first and then by NaClO for 1min was proposed.Under the conditions of 10g/L biomass,1g/L SDS,and 3%( V/V ) NaClO,the purity of PHB could be increased from 70.87% to 99.3%,the yield was 89%,and the molecular weight(MW) and polydisperity index(pI)of PHB was 1 108 000 and 1.648,resperctively (the initial MW=1 300 000,pI=1.29).
出处
《生物工程学报》
CAS
CSCD
北大核心
1998年第3期342-344,共3页
Chinese Journal of Biotechnology
