摘要
以桑椹为原料,没食子酸为标准物质研究了用福林-酚比色法测定桑椹提取液中多酚类物质含量的最适反应条件。结果表明,当福林-酚试剂与质量分数为12%的Na2CO3溶液体积比为1:2、反应温度20℃、反应时间2h时,总多酚的含量与吸光度呈良好的线性关系,测定方法的平均回收率为97.63%,相对标准偏差(RSD)为4.5%。该方法具有所用试剂量少、操作方便、灵敏度高、精密度高、稳定性高等优点,适宜桑椹样品总多酚含量的测定。
We investigated the optimum reaction conditions of Folin-Ciocalteu colorimetry for the determination of total polyphenos in 60% ethanol extract of ethanol mulberry fruits using gallic acid as a reference. When ratio of Folin-Ciocalteu reagent to 12% sodium carbonate solution （V/V） was 1：2, reaction temperature 20 ℃, and reaction time 2 h, the content of total polyphenols had a good linear relationship with absorbance at 760 nm. The average spiked recovery was 97.63%, with a RSD of 4.5%. This method can give a simple, fast, sensitive, and stable determination of total popyphenols in mulberry fruits.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2009年第18期292-295,共4页
Food Science
基金
西北农林科技大学校长基金资助项目
