摘要
ACCC 30150是由本实验室筛选的一株对黄瓜枯萎病、青椒疫病等多种土传病害具有较好防治效果的长柄木霉生防菌,为研究其在蔬菜根际的定殖情况,本试验将含绿色荧光蛋白(GFP)和潮霉素B抗性的融合基因交换整合到真核表达骨架载体pNOM102上。通过酶切鉴定和测序鉴定证明目的片段与载体片段连接正确,木霉表达载体pNOM102-HygEGFP构建成功,为下一步进行生防木霉根际定殖研究奠定基础。
ACCC 30150 was the biocontrol strain of which could effectively control many kinds of plant diseases by this laboratory screening. In order to study its colonization in the vegetables rhizosphere, the fusion gene containing green fluorescence protein （GFP） and hygromycin B resistance was exchanged and conformed to frame-vector pNOM102 which could be expressed in eukaryote. It was proved that the connection between the goal fragment and the vertor fragment was correct by identification of enzyme-cutting and sequence-measured. The successful construction of pNOMI02-HygEGFP would establish base for research on rhizosphere colonization of Trichoderma spp. in the future.
出处
《生物技术通报》
CAS
CSCD
北大核心
2009年第9期125-129,共5页
Biotechnology Bulletin
基金
中央级公益性科研院所基本科研业务费专项资金(2009年度)
关键词
载体构建
绿色荧光蛋白
潮霉素B抗性
表达载体
木霉
Vector construction Green fluorescence protein（GFP） Hygromycin B resistance Expression vector Trichoderma spp.
