摘要
运用PCR-SSCP和DNA测序的方法检测高邮鸭Ghrelin基因多态性,分析其对产蛋性能的遗传效应。结果表明:①Ghrelin基因内含子1的157 bp处发生了9 bp缺失,发现了AA、BB、AB 3种基因型,A、B等位基因频率分别为0.24和0.76。B等位基因对产蛋数、开产体重表现为正效应,BB、AB基因型个体的产蛋数显著高于AA基因型个体(P<0.05)。②内含子2的431 bp处发生了T→C的突变,发现了CC、DD、CD 3种基因型,C、D等位基因频率分别为0.41和0.59。C等位基因对开产体重具有正效应,CD基因型个体开产体重显著高于DD基因型(P<0.05)。③外显子3的909 bp处发生了A→G的突变,同时产生了苏氨酸→丙氨酸的变化,发现了EE、FF、EF 3种基因型,E、F等位基因频率分别为0.33和0.67。F等位基因对开产体重、蛋重、最大连产天数具有正效应,EF基因型个体的最长连产天数显著高于EE、FF基因型(P<0.05)。
The genetic polymorphism of Ghrelin gene was detected by PCR-SSCP and DNA se- quencing in Gaoyou duck. The genetic effects between Ghrelin gene and egg performance were analyzed. The results showed that. ①There were 9 bp deletions at 157 bp of intron 1, and the frequency of A/B allele of intron 1 was 0.24 and 0.76, respectively. The B allele gene had positive effect on egg production and body weight at first laying. BB and AB genotypes had higher egg production compared to AA genotype （P〈0.05）. ②There was T→C at 431 bp site, and the frequency of C/D allele of intron 2 was 0. 41 and 0.59, respectively. The C allele gene had positive effect on body weight at first laying egg. CD genotype had higher egg production compared to DD genotype （P〈0.05）. ③There was A→G at 909 bp site which caused Thr→Ala change, and E/F allele of exon 3 was 0.33 and 0. 67, respectively. The F allele gene had a positive effect on egg production and body weight at first laying, egg weight and highest clutch days. EF genotype had higher clutch days than EE and FF genotypes （P〈0.05）.
出处
《四川农业大学学报》
CSCD
北大核心
2009年第3期354-359,共6页
Journal of Sichuan Agricultural University
基金
国家科技支撑计划项目(2008BADB2B08)
江苏省高技术研究计划项目(BG2007323)
江苏省科技服务平台项目(BM2008170)
江苏省六大人才高峰项目
