摘要
采用ＨＰＬＣ－ＭＳ联用法测定了具有改善睡眠等特定功能的保健药品中的褪黑激素。利用质谱谱库检索和二极管阵列检测光谱图鉴定“松果体素”药品中的褪黑激素，以甲醇－水（７０∶３０）为流动相，检测波长为２７５ｎｍ。用外标法进行定量分析，方法最低检测限为０．２ｎｇ。
A rapid and precise method for the determination of melatonin in health caring medicine for specific purposes, based on high performance liquid chromatography mass spectrometry, has been developed. The compound was identified using DAD UV spectrum and mass spectrum. Hypersil ODS column, 100mm×2 1mm i.d., and mobile phase of methanol water(70∶30, V/V) were used for the assays. The quantitation was performed with external standard, and detection at 275nm. The calibration curve of melatonin was linear in the range of 5 50mg/L and the detection limit was ca. 0.2ng.
出处
《色谱》
CAS
CSCD
北大核心
1998年第5期451-453,共3页
Chinese Journal of Chromatography
关键词
褪黑激素
保健药品
HPLC
MS
分析
high performance liquid chromatography/mass spectrometry, melatonin, health caring medicine
