摘要
应用间接血凝试验(IHA)和酶联免疫吸附试验(ELISA)检测猪瘟病毒抗体,IHA试验以1∶16为判定孔,抗体效价大于或等于1∶16为抗体阳性;ELISA试验以抗体阻断率40%为判定标准,阻断率大于或等于40%为抗体阳性,同步检测了122份规模化猪场不同阶段免疫猪血清。结果ELISA检测抗体阳性率比IHA检测抗体阳性率低,阴性、阳性相符的血清数为92份,相关性为75.4%,不同抗体水平分布的血清数也不同,个别血清出现较大差异,卡方检验P值为0.13(>0.05),两种检测方法统计上无差异,研究结果为选择猪瘟病毒抗体检测方法提供参考。
Indirect hemagglutination test （IHA） and enzyme-linked immunosorbent assay （ELISA） were applied to detect the antibody of classical swine fever. The results were considered to be positive when the antibody titers were≥1： 16 by IHA tests or the block rates were≥40% by ELISA. Synchronous detection of serums were carried out among 122 large-scale pig farms at different stages with IHA and ELISA, the results of ELISA detection showed lower positive rate than that of IHA detection. The correlation was on- ly 75.4% （92 corresponding negative and positive sera） between the two methods by correlation test. The antibody level was not correlation between IHA and ELISA by correlation test,P value by chi-square test was 0.13（〉0.05）, the two detection methods had no statistical differences. The results provide a reference for the selection of classical swine fever virus antibody detection methods.
出处
《动物医学进展》
CSCD
北大核心
2009年第9期20-23,共4页
Progress In Veterinary Medicine
基金
龙岩市科技重大基金项目(2008LY02)
福建省教育厅科技项目(JA05325)
关键词
猪瘟
间接血凝试验
酶联免疫吸附试验
抗体效价
抗体阻断率
Classical swine fever
indirect hemagglutination test
enzyme-linked immunosorbent assay
anti- body titer
antibody blocking rate
