摘要
目的观察低氧环境对大鼠血清促血管生成素-1(angiopoietin-1,Ang-1)水平的影响,并探讨低氧条件下血管生长抑制剂沙利度胺对血清Ang-1水平的干预效应。方法采用低压氧舱模拟4000m海拔的高原低压低氧环境。将实验动物分为低氧组、沙利度胺预防组和沙利度胺治疗组,经不同时间的低氧处理及沙利度胺干预后,测定血清Ang-1的水平,并与对照组进行比较分析。血清Ang-1的测定采用固相双抗体夹心酶联免疫吸附实验(ELISA)法测定。结果低氧组大鼠血清Ang-1浓度呈现随低氧时间的延长而不断上升的趋势;各组与对照组间血清Ang-1浓度存在显著差异(P<0.01)。采用沙利度胺进行预防性干预后土鼠血清Ang-1水平仍呈持续增高,但增幅显著降低。经沙利度胺治疗后,治疗组血清Ang-1水平恢复到对照组水平。结论在低氧环境中,大鼠血清Ang-1浓度呈现随低氧时间的延长而不断增高的趋势;沙利度胺可部分阻止低氧引起的大鼠血清Ang-1的增高;沙利度胺对低氧引起的血清Ang-1水平增高具有治疗作用。沙利度胺在低氧引起的血管增生性疾病的预防和治疗中可能具有一定的作用。
Objective observe the serum levels of angiopoietin - 1 （ Ang - 1 ） in the hypoxic environment and to reveal the interferential effects of blood growth inhibitors - thalidomide on serum Ang - 1 in the same condition. Methods By means of low pressure oxygen chamber, the low pressure hypoxic environment at 4000 - meter - high plateau was simulated. Experimental animals were divided into three groups as the hypoxic group, the preventive group with stopped reaction and the treated group by using thalidomide. After various periods of hypoxic treatment and intervention thalidomide, Serum Ang - 1 was measured and compared with control group. Serum Ang - 1 was detected by using solid - phase double - antibody sandwich enzyme - linked immunosorbent assay （ELISA）. Results The concentrations of the serum Ang - 1 of the rats in hypoxie group increased with the extension of hypoxic time. Significant differences were found among those groups（ P 〈0. 01 ）even though preventive intervention implemented by thalidomide could not block the increase of serum Ang - 1 level, it decreased the raising extent with bigmargin. After thalidomide treatment, the serum Ang - 1 levels of treatment group returned to that of the control group without much difference. Conclusion The results above show that the concentrations of the serum Ang - 1 of the rats increase with the extension of hypoxic time. Thalidomide, in part, could block the increase of serum Ang - 1 level. Thalidomide has treatment effect on the decrease of the serum Ang - 1 level caused by hypoxic environment. For vascular proliferating disease caused by hypoxic environment, thalidomide plays a certain role in the prevention and treatment of the disease to some extent.
出处
《青海医学院学报》
CAS
2009年第3期165-168,共4页
Journal of Qinghai Medical College
