摘要
以Biolog方法为主要监测手段,结合聚类分析以及PCA分析方法,考察了添加酶液对农业废物堆肥中木质纤维素降解及微生物群落代谢能力的影响.结果表明,添加酶液使得堆料中有机质的降解率提高了4.90%;微生物群落代谢聚类结果表明,添加酶液可以改善堆肥微生物对中间代谢产物类碳源的代谢能力.通过PCA发现,添加酶液提高了堆体中微生物对双亲化合物、聚合物、氨基酸和氨基化合物等碳源的代谢能力,由此可导致有机质被更高效降解;此外,堆肥各时期群落代谢聚类结果表明,酶液对堆肥进程的影响主要表现在一次发酵的第6 d和二次发酵的第30 d,从而有效地加速了堆肥进程.
The effects of enzymes on organic material degradation and microbial communities metabolic profiles during composting process were studied using Biolog method,and together with cluster analysis and PCA.The results showed that,adding the enzyme solution in the composting could increase the degradation rate of organic material by 4.90%.The microbial community metabolic results of cluster analysis showed that when the enzyme solution was added into the compost,the carbon metabolic capability of intermediate metabolite was improved.The results of PCA indicated that when the enzyme solution was added,microbial communities enhanced the metabolic capability of miscellaneous,polymers,amino acids and amides carbon substrates,which results in the efficient degradation of organic substance.In addition,cluster analysis of each composting phase showed that the effects of the enzymes solution on microbial community metabolism were mainly observed on 6 d and 30 d,which promoted the composting process.
出处
《环境科学》
EI
CAS
CSCD
北大核心
2009年第10期3016-3021,共6页
Environmental Science
基金
国家重点基础研究发展规划(973)项目(2005CB724203)
湖南省研究生科研创新项目
国家教育部长江学者和创新团队发展计划项目(IRT0719)
国家自然科学基金青年科学基金项目(50808073)
湖南省环境保护科技项目(2007185)
