摘要
目的探讨胆固醇与胆囊动力的关系及其在胆固醇结石形成中的作用。方法构造豚鼠胆固醇结石模型，检测各组豚鼠血清胆固醇（TC）及胆汁胆固醇（BC）含量；利用灌流法加药，张力换能器记录胆囊收缩素对各组离体胆囊肌条张力的变化，并初步探讨胆固醇对胆囊肌条的作用机制。结果A组（正常豚鼠）无结石发生，B、C两组（致石饲料4周、8周）共有13例结石发生；B、C两组TC和BC升高（与A组相比P〈0．05），加入CCK后收缩振幅均增加，呈浓度依赖性，且与TC、BC相关（P〈0．05）；同A组相比，B、C及D（胆固醇孵育后胆囊肌条）三组对CCK效应值变小，起作用时间慢（P〈0．05）；CCK对胆囊离体肌条的作用可以部分被胆固醇抑制（变化为69．2％，P〈0．05），而胆固醇的影响可以部分被阿托品、尼莫地平抑制（变化分别为64．2％、62．1％，P〈0．05），不被TTX影响（P〉0．05）。结论TC及BC增高的同时胆囊肌条肌张力降低及对CCK的敏感性降低，成石率增高；胆固醇孵育后肌条肌张力降低及对CCK的敏感性降低；胆固醇可能与胆囊动力及胆固醇结石形成相关。
Objective To investigate the relationship between cholesterol, the dynamic of gallbladder and their role in cholesterol gallbladder formation by establishing an animal model of cholesterol gallstone. Methods The model of cholesterol gallstone was prepared by feeding guinea pig with stone-leading forage. The contents of serum total cholesterol （TC） and bile cholesterol （BC） of subgroup A [the normal guinea pigs], subgroup B [stone-leading forage for four weeks], subgroup C [stone-leading forage for eight weeks] were detected respectively. The effect of cholecystokini-octopeptide （CCK-8） on constrictions of extracor poreal gallbladder muscle strip was observed in subgroup A, B, C and D [gallbladder strip incubation in cho lesterol 2 hours] to probe its possible mechanism. Results No gallstone was found in subgroup A. A total of 13 cholesterol gallstone guinea pigs was seen in subgroup B and group C. With the increase of TC and BC, the amplitude of contractions was upregulated by CCK 8 in a dose-independent manner in subgroup A, B, C and D （P〈0.05）. As compared with subgroup A, there was significant change in subgroup B, subgroup C and subgroup D （P〈0. 05）. The response of CCK-8 on the constrictions of extracorporeal gallbladder muscle strip could be inhibited partly by cholesterol （change 69.2%, P〈0. 05）. The effects of cholesterol on the constrictions of extraeorporeal gallbladder muscle strip could be inhibited partially by atropine and nimodipine （changed for 64.2%, 62.1% respectively, P〈0.05）. However, it could not be affected by TTX （P〈0. 05）. Conclusion With increase in the contents of TC and BC, gallbladder muscle strip tension and it＇s sensitivity to CCK-8 are decreased and the ratio of gallstone formation is increased. Cholesterol can promote the formation of cholesterol stone by resulting in a defective gallbladder muscle strip contraction.
出处
《中华肝胆外科杂志》
CAS
CSCD
北大核心
2009年第9期689-692,共4页
Chinese Journal of Hepatobiliary Surgery
基金
基金项目：本课题由湖北省自然科学基金资助（基金编号No.2006AA412）
关键词
胆结石
胆固醇
肌条
胆囊
Cholelithiasis
Cholesterol
Muscle strip
