摘要
目的研究硒、锗联合作用对氟中毒大鼠肾脏的影响。方法将50只健康初断乳清洁级SD大鼠按体重随机分为对照组、氟染毒组、氟+硒染毒组、氟+锗染毒组、氟+硒+锗染毒组,每组10只,雌、雄各半。除对照组可自由饮用蒸馏水外,其余各组大鼠均可自由饮用100mg/L的NaF溶液。氟+硒染毒组、氟+锗染毒组、氟+硒+锗染毒组分别采用20mg/L的Na2SeO3溶液、2000mg/L的Ge-132溶液、20mg/L的Na2SeO3和2000mg/L的Ge-132溶液,按5ml/kg灌胃染毒,共饲养90d。每周称体重1次,并注意观察大鼠的活动、饮食和一般状况。染毒结束后,取出肾脏,称重,并计算其脏器系数。测定大鼠肾组织中的丙二醛(MDA)的含量和谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)的活力。并在光镜下观察肾脏的病理损伤。结果对照组大鼠活动正常,皮毛光亮;氟染毒组大鼠活动减少,皮毛不舒展,有脱毛现象;其余各组体重增长情况介于对照组、氟染毒组之间。染毒前、后各组大鼠体重间比较,差异无统计学意义(P>0.05)。与氟染毒组比较,对照组、氟+硒染毒组、氟+锗染毒组、氟+硒+锗染毒组大鼠肾脏系数均较高,差异均有统计学意义(P<0.05或P<0.01)。氟+锗染毒组大鼠肾脏系数低于对照组,差异均有统计学意义(P<0.05)。与氟染毒组比较,对照组、氟+硒染毒组、氟+锗染毒组、氟+硒+锗染毒组大鼠肾脏中SOD和GSH-Px活力均较高,MDA含量均较低,差异有统计学意义(P<0.05或P<0.01)。对照组、氟+硒染毒组、氟+锗染毒组大鼠肾脏中SOD和GSH-Px活力均低于氟+硒+锗染毒组,差异均有统计学意义(P<0.05)。氟染毒组大鼠肾脏表面较灰暗、光泽较差,肾近曲小管广泛出现明显的混浊肿胀和空泡变性,局部出现坏死崩解现象;氟+硒染毒组、氟+硒+锗染毒组和氟+锗染毒组大鼠肾近曲小管混浊肿胀和空泡变性等病变明显减轻,未见坏死崩解细胞。结论硒、锗联合染毒对氟致大鼠肾组织中的脂质过氧化反应有拮抗作用,其拮抗作用强度依次为Na2SeO3和Ge-132>Na2SeO3>Ge-132。
Objective To study the antagonistic effects of selenium and germanium （Se-Ge） in combination on the kidney damage induced by fluoride in rats. Methods Fifty SD rats were randomly divided into 5 groups, the control group （distilled water） ,fluoride group （NaF, 100 mg/L）, fluoride plus selenium group （100 mg/L NaF ＋ 20 mg/L Na2SeO3）, fluoride plus germanium group（100 mg/L NaF ＋ 2 000 mg/L Ge-132） and fluoride plus selenium and germanium group（100 mg/L NaF＋ 20 mg/L Na2SeO3＋ 2 000 mg/L Ge-132）, 10 in each group （males and females were in the same number）. The administration was conducted through gavage for 90 days. After 90 days of treatment, the kidneys were collected and the organ coefficients were calculated, MDA contents,SOD and GSH-Px activities in the tissue were determined and the histopathological examination was done. Results Fluoride decreased the organ coefficient of kidney, Se and/or Ge showed an obvious antagonism to fluoride, administration in combination was more efficient than singly. NaESeO3 and/or Ge-132 had an antagonistic effect to fluoride in the increase of lipid peroxide（MDA） and decrease of the activity of glutathione peroxidase （GSH-Px）, superoxide dismutase （SOD）. Na2SeO3 and/or Ge- 132 could prevent the pathologic damage caused by fluoride in the kidneys. Conclusion Na2eO3 and Ge-132 in combination has an obvious antagonistic effect on fluoride-induced kidney damage.
出处
《环境与健康杂志》
CAS
CSCD
北大核心
2009年第10期861-863,共3页
Journal of Environment and Health
基金
宁夏自然科学基金资助项目(NZ0570)
关键词
氟化物
硒
锗
肾脏
Fluoride
Selenium
Germanium
Kidney
