摘要
采用硫酸铵沉淀、有机溶剂分离、Sephadex G-25分子筛层析等实验方法,从文蛤肉中提取了一种低分子量的多肽,命名为Mer2.本文以溴化二苯偶氮盐(MTT)法检测Mer2对体外培养的癌细胞的抑制作用.结果表明Mer2对体外培养的人肝癌细胞株(HepG2)、宫颈癌细胞株(Hela)、胆管癌细胞株(QBC939)、肺癌细胞株(SPC-A-1和LTEP-a-2)的生长均有很强的抑制作用,且抑制效果随着Mer2含量的增高和处理时间的延长而增强,证明Mer2具有广谱的抗癌活性.其中Mer2对人肝癌HepG2细胞株抑制作用最为显著,光学显微镜观察经Mer2细胞培养液处理后的细胞形态发生明显的改变,流式细胞仪实验的结果表明处理后的细胞周期发生明显的改变,并在G0/G1期前出现凋亡峰.
An anti-cancer peptide was purified from Mercenaria （ Meretrix meretrix） by methods of fragmentation, organic precipitation and column chromatogram （Sephadex G-25 ）. Finally, the anticancer peptide was obtained and named as Mer2. The antitumor activity and stability of Mer2 were preliminarily determined by MTT method in vitro. It shows that Mer2 inhibited significantly the growth of human cancer cells including HepG2, HeLa, QBC939, SPC- A-1 and LTEP-a-2 in a dose-dependent manner in vitro, and had a more remarkable inhibitory effect on liver cancer cell strain HepG2. The cell morphology of HepG2 treated by the peptide Mer2 was changed distinctly under the LM and the proliferation of HepG2 cells through induced-apoptosis was changed clearly showed by flow cytometry.
出处
《台湾海峡》
CAS
CSCD
北大核心
2009年第4期472-476,共5页
Journal of Oceanography In Taiwan Strait
基金
厦门市科技计划资助项目(3502Z20063021)
厦门市卫生局资助项目(WSK0602)
厦门大学科技创新工程基金资助项目(XDKJCX20043001)
关键词
海洋生物学
细胞
文蛤多肽
抗癌
MTT比色法
凋亡
marine biology
cell
Meretrix meretrix peptide
antitumor
MTT colorimetry
apoptosis
