摘要
目的:为掌握黄芪根腐病菌毒素滤液产生条件和探讨黄芪根腐病菌致病机制,以及为利用毒素进行种质抗原筛选奠定理论基础。方法:用培养皿滤纸发芽法和胚根生长抑制法研究了10株来源不同的黄芪根腐病菌茄病镰刀菌Fusarium solani毒素滤液的生物活性和专化性。结果:黄芪根腐病菌F.solani在不同培养条件下产生的毒素滤液对黄芪胚根均有较强的抑制作用。随着培养时间的延长,胚根抑制率逐渐增高,第12天时毒素滤液的抑制作用最强,抑制率在52.0%～92.0%。5～35℃下病菌所产毒素滤液均具有较强抑制作用,以25℃时毒素滤液的抑制率最高,其次为20,30℃。PSC培养液所产毒素滤液的抑制率较高,其次为PDB。光照条件对毒素滤液生物活性物质的影响不显著,但24h连续黑暗更有利于毒素滤液生物活性物质的产生。所有条件下产生的毒素滤液经灭菌后对黄芪胚根仍有很强的抑制作用,表现出较高的热稳定性。供试菌株HQM40培养12d后产生的毒素滤液对4种供试植物种子的胚根都具有抑制作用。结论:10株黄芪根腐病菌在不同培养条件下产生的毒素滤液生物活性强,对热稳定;产生毒素滤液的最佳条件是PSC培养液、25℃,24h黑暗条件下培养12d。HQM40菌株所产毒素滤液所含生物活性物质为非专化性毒素物质。
Objective：To study the culture-filtrate producing condition of Fusarium Solani isolated from Astragalus root and explore the mechanism Astragalus root rot disease caused by,in order to find theoretical support for screening resistant germ plasma via mycotoxin. Method：The method of germinating seeds in petri dish with filter paper and inhibition method for embryo growth were used to study the biological activity and the specialty of cultural filtrate of 10 F. solani isolates. Result：The toxin produced by F. solani had strong inhibition effect in the different nutrient media,at different temperatures and under different light conditions. With extension of culturing time,embryo inhibition rate went up gradually with the strongest inhibition at the 12th day and the inhibition ratio between 92.0%-52.0%. The toxin produced at 5 ℃ to 35 ℃ inhibited embryo germination of Astragalus differently with the strongest at 25 ℃,and next to it at 20,30 ℃. The impact of light on bioactive substances of the toxin was not statistically distinctive,but the 24-hour darkness was benefit to toxin production. PSC had a stronger inhibition rate than the other nutrient media,next to it was PDB. After autoclaving,the toxin still kept toxic to embryo of Astragalus,which indicated that the toxin was tolerant to high temperatures. Conclusion：The toxin produced by F. solani at different growing condition had strong biological activity,was tolerant to high temperature. The best condition for F. solani to produce toxin was that it was cultured in PSC liquid medium,in dark,at 25 ℃ for 12 d. The toxin produced by isolate HQM40 was non-host specific toxin.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2009年第20期2571-2576,共6页
China Journal of Chinese Materia Medica
基金
甘肃省科学事业费项目(甘科计[2001]21号)
甘肃省教育厅项目(032-08)
甘肃农业大学科技创新基金(GAU-CX0504)
关键词
黄芪根腐病
茄镰孢
毒素滤液
毒性
胚根
Astragalus root rot disease Fusarium solani culture filtrate toxicity radicle
