摘要
目的：了解肺纤维化大鼠肺局部细胞因子水平及其与肺泡巨噬细胞的关系。方法：对比观察支气管肺泡灌洗液（ＢＡＬＦ）和肺泡巨噬细胞（ＡＭ）培养上清肿瘤坏死因子－α（ＴＮＦ－α）、血小板源生长因子（ＰＤＧＦ）的动态变化。二种细胞因子检测分别采用ＥＬＩＳＡ和生物活性法。结果：①ＢＡＬＦ中ＴＮＦ－α于第１ｄ增高，第３ｄ达高峰，其高峰时间先于ＡＭ源ＴＮＦ－α；ＢＡＬＦ中ＴＮＦ－α与其中中性粒细胞数呈正相关；②ＢＡＬＦ中ＰＤＧＦ与ＡＭ源ＰＤＧＦ同步于第７ｄ达高峰，与ＡＭ总数呈正相关。结论：ＡＭ释放高水平ＴＮＦ－α、ＰＤＧＦ；ＢＡＬＦ中也存在ＴＮＦ－α、ＰＤＧＦ，且呈动态变化；ＢＡＬＦ中ＴＮＦ－α可能还来自ＡＭ外的其它细胞，如ＰＭＮ，而ＢＡＬＦ中ＰＤＧＦ则可能主要来源于ＡＭ。
Objective:The purpose of this article was to delineate the changes of cytokines in lung foci and the correlationship among alveolar macrophage (AM) derived TNF α, platelet derived growth factor (PDGF), and the same cytokines in pulmonary focuses. Methods:We compared the AM derived TNF α, PDGF and BALF TNF α and PDGF; TNF α and PDGF in AM supernatant and BALF were measured with ELISA and bioassay respectively. Results: (1) TNF α in BALF significantly increased on 3rd ady and decreased instantly to normal levels after 7th day; AM derived TNF α increased on 3rd day, peaked on 7th day and then remained at higher level till the 28th day. (2) The peak of BALF TNF α preceded that of AM derived TNF α. (3) A good positive correlation was found between BALF TNF α and neutrophil percentage. (4) BALF PDGF peaked on 7th day and gradually decreased to normal, which was parallel to the changes of AM derived PDGF; PDGF in BALF correlated well with the total AM count. Conclusions: TNF α PDGF in AM supernatant and BALF exhibited consecutive changes in this model, other immune effector cells might also contribute to the presence of TNF α in BALF besides AM. PDGF in BALF might mainly originated from AM.
出处
《中国医科大学学报》
CAS
CSCD
北大核心
1998年第5期483-485,共3页
Journal of China Medical University
关键词
肺纤维化
炎性细胞因子
对比研究
tumor necrosis factor α (TNF α)
platelet derived growth factor (PDGF)
bronchoalveolar lavage fluid (BALF)
