摘要
目的建立一种测定姜黄原药材中主要成分姜黄素、去甲氧基姜黄素、双去甲氧基姜黄素含量的新方法。方法以醋酸地塞米松为内标,采用Welchrom-C18柱(4.6mm×250mm,5μm),乙腈-3%醋酸(50:50)为流动相,检测波长260nm,流速1mL·min-1。结果姜黄素在12.6～201.6mg·L-1(r=0.9998),去甲氧基姜黄素在4.6～73.6mg·L-1(r=0.9990),双去甲氧基姜黄素在4.2～67.2mg·L-1(r=0.9999)内线性关系良好;平均回收率为100.3%(RSD=1.2%),101.1%(RSD=1.2%),101.2%(RSD=0.9%)。结论方法准确、可靠,可作为姜黄原药材或含有姜黄的中成药的质量控制方法。
OBJECTIVE To establish a HPLC method for the determination of curcumin,demethoxy-curcumin, bisdemethoxycurcumin in Turmeric. METHODS Dexmethasone acetate was internal standard. The determination by RP-HPLC was carried out using welchroll-C18 column （4.6 mm×250 mm, 5 μm）, with the mobile phase of acetonitrile-3% acetic acid （50 ： 50） at a flow rate of 1 mL·min^-1 and the detection wavelength of 260 nm. RESULTS The calibration curves were linear in the range of 12.6-201.6 mg·L^-1 （r=0.999 8） for curcumin, 4.6-73.6 mg·L^-1 （r=0.999 0） for demethoxycurcumin and 4.2-67.2 mg·L^-1 （r=0.999 9） for bisdemethoxycurcumin. The average recoveries were 100.3% （RSD=1.2%）, 101.1%（RSD=1.2%） and 101.2% （RSD=0.9%）, respectively. CONCLUSION The method can be applied in quantitive determination of Turmeric or preparations contained Turmeric, with accuracy and reliability.
出处
《中国药学杂志》
CAS
CSCD
北大核心
2009年第18期1423-1425,共3页
Chinese Pharmaceutical Journal
基金
科技部创新基金项目(06C26211200048)
