摘要
背景与目的:观察外源性神经生长因子(β-NGF)对人胰腺癌MIAPaCa-2细胞增殖及细胞周期的影响,为深入探讨胰腺癌侵袭转移及调控机制提供实验依据。材料与方法:将不同浓度的β-NGF和酪氨酸激酶特异性抑制剂K252a作用于体外培养的MIAPaCa-2细胞不同时间,应用克隆平板实验、MTT实验和流式细胞术检测外源性β-NGF对MIAPaCa-2细胞增殖及细胞周期的影响。结果:经β-NGF处理的MIAPaCa-2细胞平均克隆形成率均明显高于对照组(P<0.05),100ng/mlβ-NGF克隆形成率最高。随着β-NGF浓度的增加和培养时间的延长,吸光度OD值逐渐增加,100ng/ml浓度组培养72h时OD值达最高,增殖率达48.68%。而随着K252a浓度的增加,MIAPaCa-2细胞在24、48、72h和96h时细胞OD值与对照组相比逐渐降低(P<0.05),100ng/ml作用72h组细胞OD值抑制率最大,为18.05%。β-NGF诱导MIAPaCa-2细胞G0/G1期百分比升高,而S期、G2/M期百分比降低,其诱导作用的最佳质量浓度是100ng/ml,最佳时间为72h。-NGF具有促进胰腺癌MIAPaCa-2细胞增殖的作用。
BACKGROUND AND AIM：To investigate the effect of nerve growth factor（β-NGF） on the proliferative ability and cell cycle of human pancreatic carcinoma cells MIA PaCa-2.MATERIALS AND METHODS：Colony forming efficiency of MIA PaCa-2 cells treated with β-NGF was higher than normal group（P〈0.05）,especially with the 100 ng/ml β-NGF group.Absorbence gradually increased,along with β-NGF concentration enhancement and culture time prolongation.Proliferation rate reached 48.68% when cultured 72 hours in 100 ng/ml β-NGF .group. However, absorbence gradually decreased in K252a group at 24, 48, 72 h and 96 h.Inhibition rate was reduced to 18.05% in 100 ng/ ml K252a group .Effect of different concentrations of β-NGF and K252a on MIA PaCa-2 cells proliferation was evaluated by MTT and Flow Cytometry. RESULTS. β-NGF promoted MIA PaCa-2 proliferation and growth which were markedly inhibited by K252a（P 〈 0.05）. CONCLUSION：β-NGF could enhance the proliferation of MIA PaCa-2 pancreatic carcinoma cells.
出处
《癌变．畸变．突变》
CAS
CSCD
2009年第5期334-338,共5页
Carcinogenesis,Teratogenesis & Mutagenesis
基金
国家自然科学基金青年科学基金项目(30801051)
日照市人民医院博士科研启动基金资助项目
关键词
胰腺癌
神经生长因子
细胞培养
流式细胞仪
增殖
pancreatic carcinoma
nerve growth factor（β-NGF）
cell culture
flow cytometry
proliferation
