摘要
对21种向日葵细胞质雄性不育(CMS)品系的线粒体基因组atpA基因位点处的DNA分子变异进行了分析和研究。首次通过基因组DNA及mtDNA与一含有部分atpA基因和orf H522的4.1kb探针Southern分子杂交分析及mtDNA限制性内切酶图谱分析证明,在atpA基因位点区域有4种DNA序列存在于21种细胞质雄性不育品系中。首次指出在向日葵CMS品系中即使细胞质来源相同,不育基因却有多种存在形式,并通过实验结果推测了导致细胞质雄性不育的机理。
Molecular variation at atp A gene loci of mitochondrial genome was analised and characterized in 21 CMS lines of sunflower. Southern blot was applied to genomic DNA and mtDNA using a fragment containing partial atp A gene and off H522 as a probe (4.1kb). After Southern analysis and mtDNA restriction pattern comparison, four kinds of DNA sequences were first described in 21 CMS lines. The results also indicated that variation may exist even they had some cytoplasmic source. The mechnisms of CMS were also discussed.
基金
国家自然科学基金(39400083)~~
