摘要
目的:探讨Bmi-1基因的表达与大肠癌病理学特征的相关性及其在大肠癌发生发展中可能的作用机制。方法:收集我院50例大肠癌组织标本,50例正常组织作为对照。利用实时荧光定量PCR(FQ-PCR)法检测标本中Bmi-1 mRNA的表达,同时利用免疫组化法检测Bmi-1和P16蛋白的表达,并结合大肠癌的临床病理学资料,分析Bmi-1基因的表达与大肠癌临床病理特征之间的关系。结果:Bmi-1mRNA及蛋白在大肠癌组织中的表达明显高于正常组织(P<0.05),免疫组化提示大肠癌组织高表达Bmi-1蛋白的同时伴有P16蛋白的低表达(P<0.05)。Bmi-1的表达与肿瘤体积大小、Dukes分期、淋巴结转移、远处转移和P16蛋白的表达水平密切相关(P<0.05),而与患者性别、年龄、血清CEA、血清CA19-9、P53等无相关性(P>0.05)。结论:Bmi-1癌基因可能通过抑制P16蛋白的表达而促进大肠癌的发生发展,检测其表达可判断大肠癌的预后。
Objective To investigate the relationship between Bmi-1 expression and the pathologic features of colorectal carcinoma and the possible role of the expression in the genesis and development of the malignancy. Methods We collected 50 samples of colorectal cancer tissue and 50 samples of normal controlled tissue to detect both Bmi-lmRNA expression by real-time, fluorescence-based quantitative PCR and the expressions of Bmi-1 and p16 protein using immunohistochemistry. Then we analyzed the association of Bmi-1 expression with the pathologic features of the tumor. Results Significantly higher expression levels of Bmi-1 gene and protein and a lower expression of pI6 protein were detected in the cancer tissue than in the normal tissue （P 〈 0.05 for both comparisons）. Bmi-1 expression was closely associated with tumor size, Dukes＇ stages, lymph node involvement, distant metastasis, and P16 expression （P 〈 0.05） ; but not associated with sex, age, serum CEA, CA19-9 level, or P53 overexpression （P 〉 0.05）. Conclusions Bmi-1 gene may accelerate the genesis and growth of eolorectal carcinoma by suppressing the expression of P16 protein. Determination of Bmi-1 expression can predict prognosis.
出处
《实用医学杂志》
CAS
北大核心
2009年第21期3589-3591,共3页
The Journal of Practical Medicine
