摘要
以BtR05为原始菌株,通过紫外诱变,获得一株对朱砂叶螨成螨有较高毒力的突变株63号.63号菌株的生长速度快,繁殖力强,LB摇瓶培养48 h后显微计数为4.88×109cfu.mL-1.63号发酵液的杀螨活性是原始菌株的1.33倍.通过斜面传代培养,该菌株能稳定遗传10代.
By using BtR05 as the original strain, mutant strain 63 having higher toxicity to adult mites of Tetranychus cinnabarinus was obtained by UV mutation. The strain grew faster and bred stronger than parental strain, its microscope counting number was 4.88 ×10^9 cfu. mL^-1 after shaking cultivation 48 h in LB liquid. The toxicity to adult mites of its fermentative broth was 1.33 times as much as BtR05. The strain could transmit 10 generations steadily through slant subculture.
出处
《福建农林大学学报（自然科学版）》
CSCD
北大核心
2009年第5期471-474,共4页
Journal of Fujian Agriculture and Forestry University:Natural Science Edition
基金
山东省科技攻关项目(2006GG2209002)
山东省自然科学基金资助项目(Y2006D18)
关键词
苏云金芽孢杆菌
紫外诱变
朱砂叶螨
毒力测定
遗传稳定性
Bacillus thruringiensis
UV mutation
Tetranychus cinnabarinus
toxicity determination
transmit stability
