摘要
从龙眼子叶胚中分离得到一个大量表达的表达序列标签(EST),该EST编码序列与金鱼草3-磷酸甘油醛脱氢酶(GAPDH)基因的同源性为84%,利用cDNA末端快速扩增(RACE)技术成功克隆了龙眼GAPDH基因全长序列.序列分析表明,龙眼GAPDH基因的cDNA全长为1395 bp,包括一个长1008 bp、编码336个氨基酸的开放阅读框(ORF),5′端非编码区(UTR)长71 bp,3′-UTR长316 bp.龙眼GAPDH基因编码的氨基酸序列与水稻、葡萄、小果野蕉、拟南芥、银杏等GAPDH基因的氨基酸序列同源性均高达85%以上,该基因在GenBank中的登录号为FJ694011.
On the basis of one cDNA-AFLP expressed sequence tag （EST） from longan （Dimocarpua longan Lour. ） cotyledon embryos which is 84% homologous to Antirrhinum majtas, the full length cDNA of glyceraldehyde-3-phosphate dehydrogenase （GAPDH） gene was cloned by rapid-amplification of cDNA ends （RACE）. The gene consisted of 1395 bp encoding a protein of 336 amino acids, with 71 bp and 316 bp at 5＇ - UTR and 3＇ - UTR, respectively. The amino acid sequence comparison with the GAPDH gene of Oryza sativa, Vitis vlnifera, Musa acuminata, Arabidopsis thaliana, and Ginkgo biloba showed that identity was all higher than 85%. The sequence was accepted and released by GenBank （accession number： FJ694011 ）.
出处
《福建农林大学学报（自然科学版）》
CSCD
北大核心
2009年第5期507-511,共5页
Journal of Fujian Agriculture and Forestry University:Natural Science Edition
基金
国家自然科学基金资助项目(30370999)
福建省自然科学基金资助项目(2009J01084)
关键词
龙眼
子叶胚
3-磷酸甘油醛脱氢酶
基因克隆
序列分析
longan
cotyledon embryo
glyceraldehyde-3-phosphate dehydrogenase
gene cloning
sequence analysis
