摘要
目的研究黄色素（ＳＹ）对血管平滑肌细胞（ＶＳＭＣ）增殖的作用及其作用机制。方法以培养的家兔ＶＳＭＣ为材料，采用细胞计数和３２Ｐ掺入法，研究了ＳＹ对ＶＳＭＣ的增殖及ＶＳＭＣ中酪氨酸蛋白激酶（ＴＰＫ）活性的影响。结果ＳＹ对血清刺激的ＶＳＭＣ的增殖具有抑制作用，且呈剂量和时间依赖性。１．０ｇ／Ｌ达到最大抑制，抑制率为５４．６％；４ｄ（１．０ｇ／ＬＳＹ）达到最大抑制，抑制率为５２．０％，超过４ｄ抑制率下降。ＳＹ抑制血清刺激的ＶＳＭＣ中ＴＰＫ的活性，在一定浓度范围内，随着ＳＹ浓度的升高，ＴＰＫ的活性下降，１．０ｇ／ＬＳＹ作用３ｄ达到最大抑制，抑制率为９７．８％。结论ＳＹ对血清刺激的ＶＳＭＣ的增殖具有抑制作用。
Objective To study the effect of safflower yellow(SY) on the proliferation of vascular smooth muscle cells (VSMC) and the underlying mechanism.Methods The effects of SY on the proliferation of VSMC and the tyrosine protein kinase (TPK) activity in VSMC were studied by means of cell count and 32 P-incorporation in cultured rabbit VSMC of passages 4 ～ 8.Results The proliferation of serum-stimulated VSMC was inhibited by SY in a dose- and time-dependent manner.The maximum inhibition rate (54.6%) was obtained on the 3rd day of incubation when SY was increased to 1.0g/L.When SY was set at 1.0g/L,the inhibition reached its climax (52.0%) on the 4th day.The TPX activity in serum-stimulated VSMC,which was also inhibited by SY,decreased with the increasing concentration of SY within a certain a certain range,with the maximum inhibition rate (97.8%) attained in the presence of SY 1.0 g/L on the 3rd day.Conclusion SY inhibits the proliferation of serum-stimulated VSMC,which may involve the role of TPK.
出处
《徐州医学院学报》
CAS
1998年第5期357-359,共3页
Acta Academiae Medicinae Xuzhou
关键词
血管
平滑肌细胞
红花黄色素
细胞增殖
Vascular smooth muscle cell Safflower yellow Tyrosine protein kinase
