摘要
用ＡＰＡＡＰ法，ＥＬＡＳＩ法检测３０例正常健康人群和２５例大肠癌病人的外周血Ｔ细胞亚群（Ｔ亚群）和血浆肿瘤坏死因子（ＴＮＦ－α）并对其手术前后进行动态观察．结果：大肠癌患者术前ＣＤ３，ＣＤ４低于正常，ＣＤ８增高；ＣＤ４／ＣＤ８比值下降，ＴＮＦ－α水平增高；手术后第７ｄ，ＣＤ３，ＣＤ４改变不明显，ＣＤ８增高，ＣＤ４／ＣＤ８仍低下，ＴＮＦ－α有所下降，但仍持续较高水平；第１５ｄ，ＣＤ３，ＣＤ４，ＣＤ４／ＣＤ８回升，ＴＮＦ－α下降近正常．通过Ｔ亚群及ＴＮＦ－α动态测定．结果表明，肿瘤患者机体存在免疫抑制，经减瘤后免疫抑制逐渐恢复，临床上可作为采用免疫治疗。
Before and after the patient with large intestine carcinoma had operation,APAAP and ELISA were used to test the levels of T-cell subgroup and TNF-αon case group (25 cases) and control group (30 persons).The results revealed that before operation,the serum CD 3,CD 4 levels in case group was lower than control group,the ratio of CD 4/CD 8 was lower,but CD 8 and TNF-αwere higher.The seventh day after operation,there was no obvious change of CD 3,CD 4 observed,the ratio of CD 4/CD 8 were still lower,CD 8 was higher,TNF-α in serum decreased lightly but still higher than normal level.The fifth day after operation,CD 3,CD 4,ratio of CD 4/CD 8 increased gradually,and TNF-α level decreased to near normal level.The result suggested that immunity depression existed in the patient with large intestine carcinoma through dynamic observation of T-cell subgroup and TNF-α level.T-cell subgroup and TNF-αcould be reference indicators of prognosis for the patient with carcinoma.
出处
《昆明医学院学报》
1998年第4期53-55,共3页
Journal of Kunming Medical College
