摘要
目的研究树舌多糖(ganoderma applanatum polysaccharides,GAPS)对人胃癌MGC-803细胞形态、细胞增生和PDGFR-β表达的影响,探讨树舌多糖抗肿瘤作用机制。方法倒置荧光显微镜观察细胞形态;MTT法检测树舌多糖对胃癌细胞MGC-803增生抑制作用;荧光显微镜和流式细胞仪检测树舌多糖作用后MGC-803细胞的PDGFR-β表达的情况。结果树舌多糖组细胞形状不规则,呈梭形或圆形、细胞间隙增大折光性差,生长状态差。树舌多糖对胃癌MGC-803细胞具有增生抑制作用,且呈时间及浓度依赖性。树舌多糖能下调MGC-803细胞PDGFR-β的表达,荧光显微镜和流式细胞仪检测结果一致,与细胞对照组比较有差异(P<0.05)。结论树舌多糖能抑制胃癌MGC-803细胞的增生,下调PDGFR-β的表达。
Objective To study the effects of ganoderma applanatum polysaccharides（GAPS） on cell morphology, proliferation and PDGFR -βexpression in cell lines MGC - 803 ,and to explore its potential mechanisms of anti - tumor of GAPS. Methods Cell morphol- ogy was observed by inverting microscope. MTT assay was used to investigate the inhibitory effect of GAPS on MGC - 803. Expressions of PDGFR -βwas analyzed by flow cytometry （ FCW）. The fluorescence intensity of expressions of PDGFR -βwas observed by fluorescence microscope. Results Cells of GAPS group were irregular shaped and grew poorly. GAPS inhibited the proliferation of MGC - 803 cells in dose - dependent and time - dependent manners . After MGC - 803 cells were treated with GAPS for 72h, GAPS could down - regulate expression of PDGFR -βobserved by fluorescence microscope which was consistented with the results of FCW with statistic significance difference as compared with control group （ P 〈 0.05 ）. Conclusion GAPS can inhibit the proliferation of gastric carcinoma MGC - 803 cells and decrease the expressions of PDGFR -β.
出处
《医学研究杂志》
2009年第11期52-54,F0003,共4页
Journal of Medical Research
基金
黑龙江省教育厅普通高等学校骨干教师创新能力资助计划(10546072)
