摘要
目的应用核素药物"99Tc-MDP"与中草药方"骨密Ⅲ号"联合治疗以及分别治疗由类固醇诱发的骨质疏松动物模型,并进行分析探讨。方法实验分为2个阶段:①由药物盐酸地塞米松肌肉注射6周,制作骨质疏松动物模型,并且进行骨量、骨密度、骨生物力学检测、核素骨显像比值(ROI)及骨细胞学等检测分析,确立骨质疏松模型成立。②在骨质疏松模型的基础上,设立单独"99Tc-MDP"治疗组、"骨密Ⅲ号"治疗组和"99Tc-MDP"联合"骨密Ⅲ号"治疗组,总疗程时间为16周,并且经上述相同的检测方法判断疗效。结果第1阶段:由药物盐酸地塞米松诱导制作的骨质疏松动物模型组与正常组比较,骨计量下降72.51%,腰椎骨密度下降26.51%,股骨头骨密度下降25.82%,骨生物力学下降30.24%(数据存在统计学意义),证实骨质疏松模型成立。第2阶段:经单独"99Tc-MDP"治疗组、"骨密Ⅲ号"治疗组和"99Tc-MDP"联合"骨密Ⅲ号"治疗组治疗后分别与骨质疏松对照组比较:骨计量分别上升100.76%、43.16%、166.88%;腰椎骨密度分别上升22.90%、14.81%、28.28%;股骨头骨密度上升22.44%、14.19%、30.03%;生物力学上升17.70%、1.82%、26.94%;核素骨显像(ROI)差值降低12.33%、22.91%、16.31%(除单独"骨密Ⅲ号"治疗组的生物力学与骨质疏松对照组比较无统计学意义外,其他各组比较结果数据均P<0.01),在细胞学上也有较明显的改变。结论采用"99Tc-MDP"联合"骨密Ⅲ号"治疗类固醇性兔骨质疏松动物模型后的骨计量、骨密度、骨生物力学、核素骨显像(ROI)、及骨细胞学等改善比单独采用"99Tc-MDP"治疗或"骨密Ⅲ号"治疗更明显,存在深入探讨的价值。
Objective The purpose of this study was to assess the value of ^99Tc-MDP combined with traditional Chinese medicine GUMI Ⅲ to the treatment of animal Models of steroid-induced osteoporosis. Methods The experiment was divided into two stages. In stage 1, the osteoporosis animal model was constructed by intramuscular injection of dexamethasone hydrochloride for 6 weeks. Subsequently, the animal model was studied with bone mass, bone mineral density, bone biomeehanical, the ratio of bone scintigraphy （ROI） and bone cellular pathology was examined to establish the osteoporosis model. In stage 2, based on the osteoporosis animal model, the animals were divided into ^99Tc-MDP treatment group, GUMI Ⅲ treatment group and ^99 Tc-MDP combined with GUMI Ⅲ treatment group. After a course of 16 weeks, the above-mentioned detection methods were re-examined for three group animal model. Results Compared with the normal control group, the osteoporosis animal model declined in bone mineral mess for 72.51%, in bone density to lumbar spine and femur head bone for 26.51% and 25.82% respectively and in bone biomechanics for 30.24% （The data is statistically significant）, therefore the osteooorosis model was proved the successful establishment. In stage 2, ^99Tc-MDP treatment group, GUMI Ⅲ treatment group and 99Tc-MDP combined with GUMIⅢ treatment group increased in bone morphology respectively for 100.76%, 43.16% and 166.88%, in lumbar spine bone density for 22.90%, 14.81% and 28.28%, in femoral bone density for 22.44%, 14.19% and 30.03%, in biomechanics for 17.70%, 1.82% and 26.94%, declined in radionuclide bone scintigraphy value（ROI） for 12.33%, 22.91% and 16.31% in contrast to the osteoporosis animal model group. In the ^99Tc-MDP treatment group and the ^99Tc-MDP combined with GUMIm treatment group, there is an obvious improvement in the above parameters compared with the normal control group but GUMI Ⅲ treatment group shows no difference with normal control group. Conclusions ^99Tc-MDP combined with GUMI Ⅲ shows more perfect curative effect in increasing the bone mass, bone mineral density, bone biomechanics, bone scintigraphy （ROI） and bone cytology to the treatment of osteoporosis rabbit model than the use of ^99Tc-MDP or GUMIm treatment significantly. However, there still need for further study.
出处
《中国骨质疏松杂志》
CAS
CSCD
2009年第11期805-809,796,共6页
Chinese Journal of Osteoporosis
