摘要
【目的】从Genbank数据库中选择与新疆沙冬青(Ammopiptanthus nanus)亲缘关系最近的同属蒙古沙冬青(Ammopiptanthus mongolicus)抗冻蛋白(AFP)基因AY590122、AY843521、AY843522的mRNA序列,设计扩增新疆沙冬青AFP基因保守区引物。【方法】以新疆沙冬青为研究材料,提取其总RNA,利用RT-PCR技术克隆出新疆沙冬青AFP基因的保守区域。【结果】序列分析表明,新疆沙冬青与蒙古沙冬青的保守区域同源性达98.15%。【结论】得到新疆沙冬青的抗冻蛋白的保守区域。
【Objective】A pair of primers were designed according to the three antifreezing proteins（AFP） gene of Ammopiptanthus mongolicus（Genbank accession number is AY590122,AY843521,and AY843522,respectively）.【Method】Total RNA of Ammopiptanthus nanus was extracted using Trizol,which then was used as a template to amplify conservative region of AFP gene by RT-PCR in Ammopiptanthus nanus.【Result】The result showed that the conservative region of AFP gene of Ammopiptanthus nanus had high homology and similarity（98.15%） to the AFP gene of Ammopiptanthus mongolicus.【Conclusion】The above result could also set the base for the complete AFP gene of Ammopiptanthus nanus
出处
《新疆农业科学》
CAS
CSCD
北大核心
2009年第6期1307-1310,共4页
Xinjiang Agricultural Sciences
基金
国家教育部科学技术研究重点项目(206170)
