摘要
目的:探讨β-胡萝卜素(β-C)对大鼠急性放射性损伤小肠黏膜结构和屏障功能保护作用的影响。方法:将40只大鼠随机分为四组,即对照组(C组)、单纯照射组(R组)、β-C 5 mg/(kg.d)组(T1组)和β-C 10 mg/(kg.d)组(T2组)。连续灌胃14 d后,R组、T1组、T2组用直线加速器,以9 Gy剂量进行全腹一次性照射,第4天处死大鼠,测定血浆二胺氧化酶(DAO)活性和内毒素浓度,留取空肠组织,观察肠黏膜形态,测定绒毛隐窝轴(CVA)长度。结果:R组大鼠体质量增长缓慢,CVA明显短于C组(P<0.01),肠黏膜损伤严重(P<0.008 3);血浆内毒素浓度较C组显著升高(P<0.01),两组间DAO活性无显著性差异。T1组大鼠CVA明显长于R组(P<0.01),肠黏膜损伤轻于R组(P<0.008 3);血浆内毒素浓度较R组明显降低(P<0.01);两组间DAO活性无显著性差异(P>0.05)。T2组大鼠体质量较T1组显著增长(P<0.01),CVA明显长于T1组(P<0.01),肠黏膜损伤轻于T1组(P<0.008 3);血浆内毒素浓度较T1组明显降低(P<0.01);DAO活性无显著性差异(P>0.05)。结论:补充10 mg/(kg.d)β-C可减轻X线照射引起的大鼠小肠黏膜损伤,对维持黏膜屏障功能有一定的保护作用。
Objective ： To investigate effects of beta-carotene on instestine mucosa barrier function in rats damaged by X-ray radiation. Methods ： 40 female SD rats were randomized into 4 groups as the normal control group （ Group C） ,the radiation group（ Group R） ,the [5-C 5mg/（ kg ·d） group （ Group T1 ） and the β-C 10 mg/（ kg ·d） group （Group T2）. After 14 days of continuous administration of peanut oil in groups C and R or beta-carotene （2.5 mL/kg） in groups T1 and T2, the rats in groups R, T1 and T2 were radiated under a 9 Gy dose of X-ray. And then 3 days later,the rats were killed＇and the amount of diamine oxidase （DAO） and the level of bacterial endotoxin were detected. The structure and length of the crypt-villus axis （CVA） of jejunum were also observed and analyzed. Results： Obvious slow weight gain was observed in group R. Compared with group C, the CVA of group R was significantly shorter （P 〈0.01 ）, and the intestinal mucosal injury was more serious（P 〈 0. 008 3）. The plasma endotoxin concentration of group R was significantly higher than that of group C （P 〈 0.01 ）. There was no significant difference in the activities of DAO between groups R and C. There was no difference in weight gain between groups T1 and R（P〉0.05）. The CVA of group T1 was significantly longer than that of group R, but the intestinal mucosal injury was slighter（ P 〈 0. 008 3 ）. The plasma endotoxin concentration of group T1 was significantly lower than that of group R（P 〈0.01 ）. There was no significant difference in the activities of DAO between groups T1 and R （ P 〉 0.05 ）. Group T2 gained more than group T1 （ P 〈 0.01 ）. The CVA of group T2 was significantly longer than that of group T1 （P 〈 0.01 ）, and the intestinal mueosal injury was slighter（ P 〈 0. 008 3 ）. The plasma endotoxin concentration of group T2 was significantly lower than that of group T1 （P 〈 0.01 ）. There was no significant difference in the activities of DAO between groups T2 and T1 （P 〉 0.05 ）. Conclusion ： Beta-carotene may decrease the X-ray radiation damage on jejunum and maintain the normal function of gut mucosa barrier in rats.
出处
《肠外与肠内营养》
CAS
北大核心
2009年第6期346-350,共5页
Parenteral & Enteral Nutrition
基金
青岛大学自然科学科研基金资助[青大科字2005(14)]
关键词
Β-胡萝卜素
放射性肠炎
肠屏障
β-carotene
Enteritis induced by radiation
Intestine mucosa barrier
