摘要
目的:近来大量的研究证明肿瘤细胞以及肿瘤微环境中的巨噬细胞等分泌的TNF-α可促进包括乳腺癌在内的多种恶性肿瘤的转移。本研究拟在基因水平上较全面的了解TNF-α(20ng/ml)作用于乳腺癌细胞系(MCF-7)引起的转移相关基因的变化,以进一步探索TNF-α促进乳腺癌转移的机制。方法:Transwell法用于检测TNF-α作用于MCF-7细胞后引起的侵袭能力的变化;cDNA微阵列分析用来检测TNF-α作用于MCF-7细胞后转移相关基因的变化。结果:低剂量的TNF-α可提高MCF-7细胞的侵袭能力,cDNA微阵列分析显示39个转移相关基因的表达发生了明显变化,TNF-α对其中6个基因之间的调节关系目前尚无相关报道。功能分析显示大多数的基因促进MCF-7细胞的转移,另外少数基因则抑制其转移。TNF-α作用后的这些基因表达变化有一定的时间依赖特点。结论:低剂量的TNF-α可通过影响一系列转移相关基因,从而增强MCF-7细胞的侵袭能力。研究中发现的一些表达变化明显的基因值得进一步的研究。
Objective：Studies have shown that TNF-α secreted by tumor cells and macrophages infiltrated in the tumor microenvi- ronment may promote the metastasis of a variety of malignant cancers,including breast cancer. The present study was designed to detect global metastasis related gene expression changes of MCF-7 cells treated by low dose TNF-α （20 ng/ml）,and to explore the mechanisms of TNF-α-induced metastasis. Methods：Transwell assay was used to investigate the invasion capability. cDNA array analysis was applied to detect the metastasis related gene expressions. Results：The invasive ability of MCF-7 cell was enhanced by low dose TNF-α. There were 39 gene expressions significantly changed after treatment of TNF-α. There were 6 genes which have not been reported to be regulated by TNF-α. Most of these genes promoted the metastasis of MCF-7 cells while the others inhibited. The gene expressions induced by TNF-α were somewhat time-dependent. Conclusions：TNF-α can enhance the invasive ability of MCF-7 cells by regulation of metastasis related genes,which may take part in every step of metastasis. Further study on some of the genes is needed.
出处
《南京医科大学学报（自然科学版）》
CAS
CSCD
北大核心
2009年第12期1658-1662,共5页
Journal of Nanjing Medical University(Natural Sciences)
基金
江苏省自然科学基金资助(30772474)
江苏省卫生厅开放基金(XK18200904)
