摘要
目的探讨蛇毒能否与鲎试剂产生凝胶反应,建立中药及有效成分抗蛇毒作用的试验途径。方法采用鲎试剂试管凝胶反应法。结果不同的蛇毒能使鲎试剂产生凝胶反应的浓度不同,眼镜蛇毒与竹叶青蛇毒为5μg/ml,五步蛇毒0.32μg/ml,蝮蛇毒37.5μg/ml,蝰蛇毒2.5μg/ml。结论蛇毒能与鲎试剂产生凝胶反应。
Objective To investigate whether venom reacts with TAL to produce gel-clot, and establish effective anti-venin assays of the active ingredients of Chinese medicine. Methods TAL-gel reaction in vitro. Results Different types of snake venom react to TAL with different concentrations of cobra and trimeresurus for 5μg/ml, acutus 0. 32 μg/ml, agkistrodon 37. 5μg/ml, viper 2.5 μg/ml. Conclusion Snake venom can reacts with TAL to produce gelclot.
出处
《蛇志》
2009年第4期261-262,共2页
Journal of Snake
关键词
蛇毒
鲎试剂
凝胶反应
snake venom
taehypleus amebocyte lysate （TAL）/limulus amebocyte lysate （LAL）/limulus reagent
get-clot reaction
