摘要
目的研究游离脂肪酸对大鼠肝脏和骨骼肌细胞酪氨酸磷酸酶蛋白量的影响，以探讨游离脂肪酸在肥胖诱发的胰岛素抵抗中的作用机理。方法分离培养Ｗｉｓｔａｒ大鼠肝脏和骨骼肌细胞，分别与软脂酸（０．２５ｍｍｏｌ／Ｌ）或油酸（０．１２５ｍｍｏｌ／Ｌ）共同孵育６～２４小时，应用Ｗｅｓｔｅｒｎ杂交方法检测ＳＨＰＴＰ２和ＰＴＰ１Ｂ的蛋白水平。结果肝脏和骨骼肌细胞的ＰＴＰ１Ｂ蛋白量显著增高（Ｐ＜００５），以２４小时为著；ＳＨＰＴＰ２蛋白量无改变（Ｐ＞００５）。结论游离脂肪酸促进大鼠肝脏和骨骼肌细胞ＰＴＰ１Ｂ表达。
Objective To determine whether that free fatty acids (FFAs) impair glucose metabolism is associated with altered insulin signaling system. Methods After skeletal muscle and hepatic cells were incubated with palmitate (0.25mmol/L) or oleate (0.125mmol/L) for 6, 12 or 24 hours, the protein abundance of SH PTP2 and PTP1B was assessed by western blot. Results SH PTP2 protein levels showed no significant change in both fatty acids treated muscle and hepatic cells at all time points. The PTP1B was significantly elevated in both liver and muscle cells incubated with FFAs at 6, 12 and 24 hours. Conclusions FFAs promote the expression of PTP1B in rat skeletal muscle and liver cells, and the elevated PTP1B may mediate the insulin resistance induced by FFAs.
出处
《中华医学杂志》
CAS
CSCD
北大核心
1998年第10期753-755,共3页
National Medical Journal of China
基金
卫生部重点课题基金
