摘要
目的探讨胃癌组织中胎盘型谷胱甘肽-S-转移酶π(GST-π)、P-糖蛋白(P-gp)、拓扑异构酶Ⅱ(Topo-Ⅱ)、胸苷酸合成酶(TS)及多药耐药相关蛋白(MRP)的表达特点及其临床意义。方法应用免疫组化SP法检测48例胃癌组织和10例正常胃黏膜组织中GST-π、P-gp、Topo-Ⅱ、TS和MRP的表达,并结合其临床病理资料进行综合分析。结果GST-π、P-gp、Topo-Ⅱ、TS和MRP在胃癌组织中的表达阳性率依次为72.9%(35/48)、56.3%(27/48)、83.3%(40/48)、41.7%(20/48)和39.6%(19/48),而在正常胃黏膜组织中其表达阳性率依次为10.0%(1/10)、0(0/10)、0(0/10)、0(0/10),其差异均有统计学意义(GST-π:P<0.01,P-gp:P<0.01,Topo-Ⅱ:P<0.01,TS:P<0.05,MRP:P<0.05);它们在胃癌组织中的表达与患者性别、年龄、肿瘤部位、大小、侵袭深度和淋巴结转移均无关(P>0.05),而与肿瘤的分化程度有关(P<0.01)。结论GST-π、P-gp、Topo-Ⅱ、TS和MRP在胃癌组织中的高表达是胃癌多药耐药产生的基础。GST-π、P-gp、Topo-Ⅱ、TS和MRP的联合检测可用于指导临床合理选择化疗方案。
Objective To detect the characteristic of multidrug resistance gene products expressions in gastric cancer tissues,including glutathione-s-transferase π （GST-π）,P-glycoprotein （P-gp）,topoisomerase Ⅱ （Topo-Ⅱ）,thymidylate synthase （TS）,and multidrug resistance related protein （MRP）,and analyze their clinical significance for the therapy of gastric cancer.Methods SP immunohistochemical stain was used to detect GST-π,P-gp,Topo-Ⅱ,TS and MRP expressions in sample of 48 gastric cancer tissues and 10 normal gastric mucosa. And their corresponding clinical data were comprehensive analyzed. Results The expressions of GST-π, P-gp, Topo-Ⅱ , TS and MRP had notable differences between the gastric cancer tissues and normal gastric mucosa （GST-π： P〈0.01 ; P-gp： P〈0.01; Topo-Ⅱ： P〈0.01; TS： P〈0.05; MRP： P〈0.05）. Positive expression rates of GST-π, Pgp, Topo-Ⅱ , TS and MRP in gastric cancer tissues were 72.9% （35/48）, 56.3% （27/48）, 83.3% （40/48）, 41.7% （20/48） and 39.6% （19/48）, and positive expression rates of them in normal gastric mueosa were 10. 0% （1/10）, 0 （0/10）, 0 （0/10）, 0 （0/10） and 0 （0/10） corresponding. Their positive expression rates were closely relevant to the degree of differentiation （P〈0.01）, but not to the patients＇ sex, age, tumour site, size of tumour, invasive depth and lymph node metastasis （P〉0. 05）. Conelusions The expressions of GST-π, P-gp, Topo-Ⅱ , TS and MRP in gastric cancer tissues exist obvious heterogeneity. Their overexpression underlie the multidrug resistance of gastric cancer. The joint detection of GST-π, P-gp, Topo -Ⅱ , TS and MRP can be looked as an important symbol for guiding its chemotherapy.
出处
《中国普外基础与临床杂志》
CAS
2009年第12期1000-1004,共5页
Chinese Journal of Bases and Clinics In General Surgery
关键词
胃癌
多药耐药
基因
化疗
免疫组化
Gastric cancer
Multidrug resistance
Gene
Chemotherapy
Immunohistochemistry
