摘要
目的研究筛选鸦胆子油脂质体(BJOL)的处方和制备工艺。方法采用薄膜-分散法制备鸦胆子油脂质体,并对其理化性质进行研究。分离法对其回收率和包封率进行测定;电子显微镜观察鸦胆子油脂质体的形态;透射电镜研究鸦胆子油脂质体的平均粒径和粒径分布;微电泳仪测定了所制得的鸦胆子油脂质体的Zeta电位;分光光度计测定脂质体的吸光度变化。结果鸦胆子油脂质体的包封率和回收率分别为93.53%和95.38%;脂质体大多为多室脂质体,形态圆整,双分子膜呈指纹状螺纹结构;平均粒径为129.7780nm;Zeta电位值为-23.7239mv。结论薄膜-分散法制备鸦胆子油脂质体工艺可行,同时具有重现性好,包封率高,质量稳定等优点。
Objective To optimize the formulation and preparation of brucea javanica oil liposome. Methods To produce brueea javanica oil liposome by thin layer dispersion technique and demonstrate its physic-chemical property. The recovery and envelopment were detected by separation method; the shape was observed under electronic microscope; the average diameter and distribution of particles was studied by transmission electron microscope; Zeta potential was tested by microiontophoresis and absorption was detected by spectrophotometer. Results The entrapment efficiency and recovery efficiency were 93.53% and 95.38%, respectively. Most of them were muhilamellar vesicles, round and small, the molecular membrane appeared wtih fingerprint spiral structure. The average size was 129. 7780 nm and Zeta value was -23. 723 9 mv. Conclusion These results suggested that thin layer dispersion technique is feasible, repeatable, high entrapment efficient and stable.
出处
《医药导报》
CAS
2010年第1期75-77,共3页
Herald of Medicine
基金
哈尔滨市科技创新人才研究专项资金项目(基金编号:2008RFQQ082)
关键词
鸦胆子油
脂质体
质量评价
Brucea Javanica Oil
Liposome
Quality evaluation
