摘要
目的:探讨编码5-脂氧合酶激活蛋白FLAP的基因ALOX5APT(-1340)G多态性与中国北方汉族人群冠心病发病的相关关系。方法:选自2006-01/2007-09在解放军沈阳军区总医院行选择性冠状动脉造影者共680例。根据造影结果分为冠心病组336例均为选择性冠状动脉造影阳性,对照组344例为造影阴性或动脉管腔狭窄<50%且临床上无相关心肌缺血证据者。在随机选择的无亲缘关系的48名中国北方汉族个体中,采用聚合酶链反应-重测序法对ALOX5AP基因进行单核苷酸多态的筛查,共发现7个多态。冠心病组和对照组受试者采用聚合酶链反应-限制性片段长度多态性方法检测ALOX5AP基因T(-1340)G多态性位点在两组间的基因型和等位基因分布。结果:ALOX5AP基因T(-1340)G3种基因型(TT型,TG型和GG型)在冠心病组分布频率分别为26.79%,51.79%和21.43%,在对照组分别为33.72%,47.38%和18.90%,两组间的基因型分布皆符合Hardy-Weinberg平衡定律,3种基因型在两组间的分布差异无显著性意义(χ2=3.90,P>0.05)。G等位基因在两组间的分布频率为47.32%和42.59%,差异无显著性意义(χ2=3.08,P>0.05)。按性别分层进行亚组分析,发现ALOX5APT(-1340)G多态的基因型和等位基因频率在冠心病组和对照组间的比较差异无显著性意义。结论:5-脂氧合酶激活蛋白基因ALOX5APT(-1340)G多态性与中国北方汉族人群冠心病发病可能无相关关系。
OBJECTIVE： To investigate the possible association between the gene ALOX5AP encoding 5-1ipoxygenase activating protein （FLAP） and coronary artery disease （CAD） in the Han population of North China. METHODS： A total of 680 cases underwent selective coronary angiography （SCA） from Shenyang General Hospital of Chinese PLA was recruited from January 2006 to September 2007. According to the results of SCA, 680 cases were divided into CAD group with angiography positive （n=336） and control group with angiography negative or the stenosis of coronary arteries 〈50% （n=344） without evidence of cardiac ischemia. Single nucleotide polymorphisms of ALOX5AP gene was screened in 48 unrelated Han individuals of North China by polymerase chain reaction （PCR）-Re-sequencing method and 7 polymorphisms were found. The genotype and allele distribution of T（-1340）G polymorphism between two groups was determined by polymerase chain reaction and restriction fragment length polymorphism （PCR-RFLP） analysis in CAD and control subjects. RESULTS： The genotype frequencies of TT, TG and GG in the ALOX5AP T（-1340）G polymorphism were 26.79%, 51.79% and 21.43% in CAD patients, 33.72%, 47.38% and 18.90% in the controls, respectively （x^2=3.90, P 〉 0.05）. The genotype distribution between two groups was in accordance with hardy-weinberg equilibrium. There are no significant differences in the distribution of three genotypes between the two groups. The frequencies of ALOX5AP G allele in cases and controls were 47.32%, 42.59%, respectively （x^2=3.08, P 〉 0.05）. Subsequent stratified analysis by gender also showed no statistical significance in the genotype frequencies and allele frequencies between the two groups. CONCLUSION： The result suggests that T（-1340）G polymorphism of the ALOX5AP gene might not be associated with CAD in the Han population of North China.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2009年第50期9974-9978,共5页
Journal of Clinical Rehabilitative Tissue Engineering Research
基金
军队"十一五"计划科技攻关课题资助项目(06G021)~~
