摘要
目的:研究吴茱萸水提液给药对大鼠内源性代谢产物的影响。方法:吴茱萸水提物按0.385 7 g.kg-1给SD大鼠连续灌胃33 d,收集尿液,通过Agilent 1200 6410三重四极杆质谱对尿液中内源性代谢物进行全扫描分析,SIMCA-P软件经过PCA,PLS-DA分析。结果:给药组与对照组相比,大鼠尿液代谢物白细胞三烯,棕榈酰乙醇胺,二十二碳六烯酸,8(R)-氢过氧亚油酸/花生酸等有明显变化。结论:吴茱萸水提物灌胃对正常大鼠机体代谢产生影响,为进一步阐释吴茱萸的药性研究工作提供依据。
Objective： To study the change of endogenous metabolites of SD rats administrated of aqueous extract of Evodiae rutaecarpa. Method： Six SD rats had been successively administrated aqueous extract of E. rutaecarpa（0. 385 7 g .kg -1 ） for 33 days. An agilent 1200 6410 triplequadrupole mass spectrometer was used for the analysis of endogenous metabolites in rat urine samples. These data was analyzed by the principal component analysis （PCA） and PLS-DA using the SIMCA-P 10.0 software. Result： The significant difference in metabolic profiles between the control group and the dosed group was well observed by PCA of the MS data. Conclusion ： The E. rulaecarpa has changed the endogenous metabolites of SD rats. This work can provide the base for the further research on the interpretation of drug property of E. rulaecarpa.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2010年第1期99-102,共4页
China Journal of Chinese Materia Medica
基金
国家重点基础研究发展计划(973)项目(2006CB504702)
