摘要
采用正交实验对山茱萸中的熊果酸进行超声波提取工艺优化,并进一步用石油醚和四氯化碳对提取液进行除杂。研究结果表明:用超声波提取熊果酸的最佳工艺条件为:80%乙醇,料液比1:20,提取时间30 min,提取温度55℃。适宜的除杂办法为石油醚洗脱3次,用量为0.2倍于提取液的体积;四氯化碳洗脱3次,用量为0.3倍于提取液的体积。
Uhrasonic extraction of ursolic acid in Cornus of officinalis was optimized through orthogonal experiments, and impurities in extract were removed using petroleum ether and carbon tetrachloride. As the results showed： the best condition for ultrasonic extraction was as follows： 80% ethanol, solid-liquid ratio 1 ： 20, temperature 55℃, supersonic wave treatment for 30 minutes. The suitable method for impurity removal was using petroleum ether to elute the extracting solution for three times with 0. 2 times volume of the extracting solution, and then using carbon tetrachloride to elute for three times with 0.3 times volume of the extracting solution.
出处
《经济林研究》
2009年第4期46-51,共6页
Non-wood Forest Research
基金
国家自然基金项目"山核桃等经济林木生物活性成分及抗氧化特性研究"(30571520)
安徽省高校拔尖人才项目
关键词
超声波提取
山茱萸
熊果酸
正交试验
除杂
ultrasonic extraction
Oornus officinalis
ursolic acid
orthogonal test
impurity removal
