摘要
采用循环伏安法在铂金电极上电聚合一层聚中性红膜,将葡萄糖氧化酶和纳米银通过静电吸附交替固定于聚中性红修饰的铂金电极表面,最后用聚中性红包埋电极,从而制得葡萄糖生物传感器。中性红能够在电极表面形成一层性能稳定并对生物分子有较强的电催化作用复合膜,加入纳米银后,显著增加葡萄糖氧化酶固定量,提高传感器的响应灵敏度。电极在葡萄糖浓度为0.5×10-8～3.5×10-6mol/L的范围内,氧化峰峰电流值与葡萄糖呈良好的线性关系,检测下限为0.25×10-8mol/L(S/N=3)。该传感器制备方法简单、灵敏度高、稳定性好,并具有抗抗坏血酸、尿酸干扰的特点。
A new approach to construct an amperometric glucose biosensor. A stable electropolymerized neutral red films are electropolymerized on the Pt electrod by the cyclic voltamemetry, glucose oxidase and colloidal Ag were immobilized onto the surface of Poly（neutral red） modified Pt elecreod by another Poly（neutral red）. electropolymer- ized neutral red films were stable,and the Ag colloids could enhance the current response to glucose and extend the linear range. The oxidation current investigated with cyclic voltammetric peak current was linear with glucose concentration ranging from 0.5 × 104 - 3.5 × 104 mol/L, with calculated limit of 0.25 × 104 mol/L （S/N=3）. The biosensor exhibited fast response, high sensitivity, fine stability. In the meantime the biosensor can effectively eliminate interference fromcommon interferents, such as ascorbic acid and oxamide and so on.
出处
《化学传感器》
CAS
2009年第4期58-61,共4页
Chemical Sensors
