摘要
目的探讨乳腺肿瘤组织中金属蛋白酶组织抑制物ＴＩＭＰ┐３及ＴＩＭＰ┐４ｍＲＮＡ表达与乳腺癌转移的关系。方法以原位杂交方法检测二甲基苯蒽（ＤＭＢＡ）诱发的大鼠乳腺原发瘤组织中此两种ｍＲＮＡ的表达水平，并观察转移组、非转移组、良性肿瘤组的表达意义。结果ＴＩＭＰ┐３及ＴＩＭＰ┐４ｍＲＮＡ均表达于肿瘤间质，乳腺癌非转移组表达水平显著高于转移组及良性肿瘤组。结论ＴＩＭＰ┐３及ＴＩＭＰ┐４ｍＲＮＡ由间质细胞产生，过度表达反映了宿主细胞对肿瘤侵袭的一种应答反应，可能是转移行为的负性调节因子。
Objective:To study the relationship between expression of tissue inhibitor of metalloproteinasesTIMP-3,TIMP-4 mRNAs and metastasis in breast carcinoma. Methods:The expression of TIMP-3 and TIMP-4 mRNAs was examined by in situ hybridization in the 7, 12dimethylbenz(α)anthracene induced breast tumors. Their significance was analysed in the metastatic(M+),nonmetastatic(M-), and benign tumor (BT) groups.Results:It was found that TIMPs signal was localized in stroma,and that the levels of their mRNA expression in M- group were significantly higher than those in M+ group as well as BT group.Conclusion:These findings indicate that TIMP-3 and TIMP-4 mRNAs were produced by stromal cells,and the overexpression of them in breast tumor represented the host response to tumor progression,and might be negative regulators in metastasis.
出处
《肿瘤》
CAS
CSCD
北大核心
1998年第5期331-333,共3页
Tumor
关键词
金属蛋白酶
组织抑制物
乳腺肿瘤
肿瘤转移
Tissue inhibitor of metalloproteinase(TIMP) Metastasis Breast cancer
